FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680914
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC113620.4238106854602572No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT106980.39904301294260097No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT96580.3602502728547055No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA94260.351596507758175No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC82180.30653724811761957No Hit
ATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTAGA75300.28087435852101184No Hit
ATCTGGGACTGAGGTGAAGAAGCCTGGGTCCTCGGTAAAGGTCTCCTGCA73180.27296660765694086No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC66450.2478632287346778No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC64370.24010468071709873No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA63210.23577779816883346No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC59060.22029800284529827No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT58820.21940278576634684No Hit
GAGTTATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCA58400.21783615587818184No Hit
GTCTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGG54040.20157304561056413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT51410.19176295845372138No Hit
CTCCTATCTTTGGTTTTGCAAACTACGCGCAGAACTTCCAGGGCAGAGTC50990.19019632856555638No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT50200.18724957234734124No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG48200.17978943002274597No Hit
GGTATCAGCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCA47590.17751408661374443No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT45790.17079995852160867No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT45690.17042695140537892No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG44500.16598816672224473No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATAAGTAA44110.16453343896894865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT43920.1638247254481121No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT43870.16363822188999722No Hit
ACTTACTACTGTCAACAGAGTTATGGTATCCCGAACACTTTCGGCCAGGG43740.16315331263889854No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC43670.1628922076575377No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG43170.1610271720763889No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43110.160803367806651No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41780.15584237316079516No Hit
CTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGGCC41640.1553201631980735No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG41570.15505905821671265No Hit
TTCAGATACTTTGCGTTCAACTGGGTGCGACAGGTCCCTGGACAAGGGCT41300.1540519390028923No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC41160.15352972904017062No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG40680.15173929488226776No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG40450.15088137851493932No Hit
CTCCTGATCTCTGCTGCATCCAGCTTGCAAAGTGGGGTCCCATTAAGGTT39670.14797192300834716No Hit
GTCTACTTGGAATTGAGCAGCCTGAGATTTGAAGACACGGCCGTCTATTA39520.1474124123340025No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC39370.14685290165965786No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG38120.1421903127067858No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG37950.14155620060919522No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37860.14122049420458843No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC37760.14084748708835867No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37530.13998957072103022No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG37490.13984036787453832No Hit
GTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGG37280.1390570529304558No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA37200.138758647237472No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA36580.13644600311684746No Hit
CAATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTA36480.1360729960006177No Hit
CCATAACTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGTTGCAGACT36350.135588086749519No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36170.13491667394030543No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC35970.1341706597078459No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA35890.1338722540148621No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC35800.1335365476102553No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG35500.13241752626156603No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC35390.1320072184337133No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA35070.13081359566177805No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC34970.13044058854554827No Hit
ATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCAAACGA34720.12950807075497386No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34390.12827714727141565No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGAC33910.12648671311351278No Hit
CCTCCACGCCATCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCCTGG33200.12383836258828146No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC33120.12353995689529763No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA33040.12324155120231385No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG32930.12283124337446111No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC32410.12089160637006632No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA31550.11768374517049035No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA31410.11716153520776869No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGG31090.11596791243583345No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACTTA30960.11548300318473476No Hit
ATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCTTGCA30870.11514729678012796No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC30860.115109996068505No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG30850.11507269535688201No Hit
CTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGA30630.11425207970117654No Hit
AGATAGGAGTGATCCCTCCCATGCACTCAAGCCCTTGTCCAGGGACCTGT30320.11309575764086427No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29940.11167833059919118No Hit
GTAGTAAGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA29530.11014900142264913No Hit
AGTCAGGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAA29370.10955219003668153No Hit
GTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACA28990.10813476299500842No Hit
CCCAAGGACACTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGT28920.10787365801364758No Hit
GCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCT28800.10742604947417188No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA28590.10664273453008935No Hit
TGAGAAGACATTCCCCTCCTGCCACCTGCTCTTGTCCACGGTTAGCCTGC28590.10664273453008935No Hit
GGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAAAGCCC28360.10578481816276092No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAG28080.10474039823731758No Hit
CATTAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC28080.10474039823731758No Hit
GTCTTGGCATTATGCACCTCCACGCCATCCACGTACCAGTTGAACTGGAC27990.10440469183271078No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27970.10433009040946484No Hit
GTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCCCCTGGCCGAAAGTG27450.10239045340507005No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG27420.10227855127020113No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG27170.10134603347962672No Hit
GTCCAGGGACCTGTCGCACCCAGTTGAACGCAAAGTATCTGAAGGTGCCT26900.10033891426580636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)