FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547953
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT148050.41728286705038087No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC147370.4153662689443744No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA117160.33021857955841016No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT113570.32010006896934656No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC98130.2765820178564936No Hit
ATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTAGA91840.25885348537593367No Hit
ATCTGGGACTGAGGTGAAGAAGCCTGGGTCCTCGGTAAAGGTCTCCTGCA91670.25837433584943204No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC87130.24557822496521234No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC79310.22353734674613784No Hit
GAGTTATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCA79000.22266360349192904No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC77960.2197323358003897No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA72320.20383584562704188No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT71080.2003408726102065No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT69780.19667678799578237No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG66090.18627642474407072No Hit
GGTATCAGCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCA64730.1824432285320578No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT62840.17711621320801038No Hit
GTCTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGG62010.17477683610803185No Hit
CTCCTATCTTTGGTTTTGCAAACTACGCGCAGAACTTCCAGGGCAGAGTC61980.17469228030923747No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT61800.17418494551647107No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATAAGTAA59670.1681814838020684No Hit
ACTTACTACTGTCAACAGAGTTATGGTATCCCGAACACTTTCGGCCAGGG57550.16220620735393057No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56680.15975408918889286No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG56670.15972590392262806No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG54890.15470892652749346No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG53830.15172128830342455No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG53660.1512421387769229No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT53300.15022746919139007No Hit
CTCCTGATCTCTGCTGCATCCAGCTTGCAAAGTGGGGTCCCATTAAGGTT52930.14918461433959243No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT52730.14862090901429642No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC52490.14794446262394118No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT52200.14712708990226195No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC50620.14267381783242336No Hit
CTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGGCC50220.1415464071818313No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49610.13982710593967845No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG49560.13968617960835444No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG48910.13785413730114238No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT48880.13776958150234797No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC48880.13776958150234797No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG48740.13737498777464074No Hit
GTCTACTTGGAATTGAGCAGCCTGAGATTTGAAGACACGGCCGTCTATTA48520.13675491191681513No Hit
ATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCAAACGA48020.13534564860357506No Hit
TTCAGATACTTTGCGTTCAACTGGGTGCGACAGGTCCCTGGACAAGGGCT47650.13430279375177742No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG47500.13388001475780542No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG46970.13238619564577098No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC46920.13224526931444697No Hit
CCATAACTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGTTGCAGACT46130.13001863327952767No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC45980.12959585428555565No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA45610.128552999433758No Hit
CAATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTA45120.12717192138678274No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG44950.12669277186028113No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG44450.12528350854704107No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA44160.12446613582536184No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA43840.1235642073048882No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC43080.1214221270687633No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA43070.12139394180249852No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC42970.1211120891398505No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC42650.12021016061937687No Hit
ATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCTTGCA42370.11942097316396243No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG42090.11863178570854799No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG41880.11803989511698719No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA41660.11741981925916156No Hit
GTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGG41600.11725070766157274No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC41480.11691248446639513No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACTTA41080.11578507381580308No Hit
AGTCAGGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAA40580.11437581050256303No Hit
CCTCCACGCCATCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCCTGG40350.11372754937847261No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGAC39660.11178276600620131No Hit
GCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCT39550.1114727280772885No Hit
GTAGTAAGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA38750.10921790677610442No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA38730.1091615362435748No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA38620.108851498314662No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG38590.10876694251586759No Hit
GGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAAAGCCC38290.10792138452792356No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA38170.10758316133274595No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC38160.10755497606648116No Hit
CTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGA37630.10606115695444669No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37150.10470826417373624No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGG36960.10417274411470502No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACTTACTACTGTCAAC36940.10411637358217542No Hit
CATTAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC36460.10276348080146494No Hit
AGATAGGAGTGATCCCTCCCATGCACTCAAGCCCTTGTCCAGGGACCTGT36320.10236888707375773No Hit
TGAGAAGACATTCCCCTCCTGCCACCTGCTCTTGTCCACGGTTAGCCTGC36080.10169244068340252No Hit
CTGCTGCATCCAGCTTGCAAAGTGGGGTCCCATTAAGGTTCAGTGGCAGT35900.10118510589063609No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG35840.10101599429304729No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCC35820.10095962376051769No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC35660.10050865950028086No Hit
GTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCCCCTGGCCGAAAGTG35590.10031136263642726No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA35480.10000132470751445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)