FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391842
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATACTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA118550.4956431068607375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT100020.41817143440076726No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC97770.40876445852192583No Hit
GTATTAGTACCTGGCTGGCCTGGTATCACCAGAAACCAGGGAAAGCCCCT86590.36202224059950444No Hit
TAATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAG73890.30892508786115475No Hit
CAATATAATACTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGAT73080.3055385765447718No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGCCTGCAGGCTAC66250.27698317865477734No Hit
GTATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGCCTGCAGGCT66240.2769413698730936No Hit
TCTCTAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGATTCAGCGGC61240.25603697903122363No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58580.2449158431033488No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC58170.24320168305431547No Hit
GTATTTATTACGTCCATCATATGAAATCATTGCCACCCACTCCAGCCCCT57170.2390208048859415No Hit
GAGTATTAGTACCTGGCTGGCCTGGTATCACCAGAAACCAGGGAAAGCCC55420.23170426809128697No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC53440.22342612931790642No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT52330.21878535455101134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50640.21171967044645926No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG45730.19119155863974294No Hit
AATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA45400.1898118688441795No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG44910.18776323854167626No Hit
ATACTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA44700.1868852541263177No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44150.184585771133712No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT43200.1806139368737567No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG42350.1770601904306388No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA39820.1664825686646526No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA39590.16552096668592658No Hit
CTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT38880.16255254318638104No Hit
ATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACT37970.1587479440531607No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36920.154358021976368No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC35880.15000990868125905No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35070.14662339736487612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA35000.14633073589308992No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA34990.1462889271114062No Hit
TCAGTAGCCTGCAGGCTGATGATGTTGCAACTTATTACTGCCAACAATAT34970.1462053095480387No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC34310.14344592995691188No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34180.14290241579502325No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG33950.14194081381629722No Hit
ATTAGTACCTGGCTGGCCTGGTATCACCAGAAACCAGGGAAAGCCCCTGA33660.14072835914746876No Hit
GTAAAAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA33230.13893058153506796No Hit
ACTTATTACTGCCAACAATATAATACTCTAGAGCTCACTTTCGGCGGAGG32720.13679833366919722No Hit
GAATTCACTCTCACCATCAGTAGCCTGCAGGCTGATGATGTTGCAACTTA32380.13537683509195006No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA31470.13157223595872972No Hit
TTTGAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG31280.13077786910673866No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG31020.12969084078296142No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT30740.1285201948958167No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC30540.1276840192621419No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30450.12730774022698824No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC29870.12488283088933133No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC29630.12387942012892156No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29220.12216526007988822No Hit
CCTTAGAGATCAGGAGCTCAGGGGCTTTCCCTGGTTTCTGGTGATACCAG28650.11978215952391505No Hit
CACCATCAGTAGCCTGCAGGCTGATGATGTTGCAACTTATTACTGCCAAC28560.11940588048876138No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28270.11819342581993293No Hit
GTATCAACGCAGAGTACGGGAGGAGTCAGACCCAGTCAGGACACAGCATG28230.11802619069319796No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27930.11677192724268576No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC27910.11668830967931829No Hit
CTAGAGTATTATATTGTTGGCAGTAATAAGTTGCAACATCATCAGCCTGC27820.11631203064416462No Hit
ATAATACTCTAGAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA27770.11610298673574591No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG27690.11576851648227601No Hit
GTTTTTTACTGTGCGAAGGGGGGACATCGGGCAGTTACAGAAACTTACTT27310.1141797827782939No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27250.11392893008819144No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTAC26760.11188029978568818No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA26090.10907911141287761No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT25750.10765761283563044No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC25310.10581802644154589No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25070.10481461568113612No Hit
GTACCTGGCTGGCCTGGTATCACCAGAAACCAGGGAAAGCCCCTGAGCTC24960.104354719082615No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG24430.10213885365337677No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG24110.10080097263949708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)