FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005112249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589789
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120700.4660611347102023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57820.22326143172281604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57750.22299113943259471No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG42010.16221398731711348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG35290.1362659274558661No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA34380.13275212768298886No Hit
GTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCACC33890.13086008165143956Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG32300.1247205853449837No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT26170.10105070335845892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)