FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052876
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC132620.4344100448233076No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT132330.43346012088273483No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA91920.3010931331636136No Hit
ATCTGGGACTGAGGTGAAGAAGCCTGGGTCCTCGGTAAAGGTCTCCTGCA84600.27711574266363914No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC81540.267092407290699No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT81000.26532358340135664No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC80280.2629651515489001No Hit
ATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTAGA78270.2563811959607924No Hit
GAGTTATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCA69760.22850584170467456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC67920.22247873808173016No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC67270.22034959821492914No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT61160.20033568346699962No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT60020.19660149970061017No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA59750.195717087755939No Hit
GGTATCAGCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCA56600.18539894840144178No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT54890.1797976727518576No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG54530.17861845682562935No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATAAGTAA54010.17691514493218852No Hit
CTCCTATCTTTGGTTTTGCAAACTACGCGCAGAACTTCCAGGGCAGAGTC52480.17190347724571847No Hit
GTCTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGG52390.1716086732641614No Hit
ACTTACTACTGTCAACAGAGTTATGGTATCCCGAACACTTTCGGCCAGGG51050.16721936953875624No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT50260.16463164570064426No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48790.15981651400187888No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG46310.15169302651008426No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG45180.14799159874164558No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45000.14740199077853147No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT44870.14697616280517126No Hit
CTCCTGATCTCTGCTGCATCCAGCTTGCAAAGTGGGGTCCCATTAAGGTT44860.14694340680722048No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC43560.14268512707361847No Hit
CTATTATTGTGCGAGAGGTCAAGGAAGGGCCGGGGATCTTGATTGGGGCC43030.14094905918222686No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC42720.13993362324575254No Hit
TTCAGATACTTTGCGTTCAACTGGGTGCGACAGGTCCCTGGACAAGGGCT42440.13901645530313056No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA42350.1387216513215735No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC42300.13855787133181957No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42030.13767345938714837No Hit
CCATAACTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGTTGCAGACT41580.13619943947936305No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG41450.13577361150600287No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT41140.13475817556952854No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT40560.13285832768838302No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG40460.13253076770887517No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG40370.1322359637273181No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40360.13220320772936733No Hit
ATGGTATCCCGAACACTTTCGGCCAGGGGACCAAGGTAGACATCAAACGA40090.13131879578469613No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG39820.13043438384002495No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG39440.12918965591789514No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG39370.12896036393223964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC39100.12807595198756844No Hit
ATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCTTGCA38640.12656917608183235No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC38590.1264053960920784No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG38460.12597956811871822No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG38410.1258157881289643No Hit
GTCTACTTGGAATTGAGCAGCCTGAGATTTGAAGACACGGCCGTCTATTA38390.12575027613306272No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA38330.12555374014535803No Hit
AGTCAGGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAA38180.12506240017609624No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA37850.12398145224372034No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACTTA37280.12211436036052561No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC36560.11975592850806913No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG36370.11913356454700419No Hit
CAATAATAGACGGCCGTGTCTTCAAATCTCAGGCTGCTCAATTCCAAGTA36260.11877324856954558No Hit
GTCTTCCTGTTCCCCCCAAAACCCAAGGACACTCTCATGATCTCCCGGAC35920.11765954463921889No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA35900.11759403264331732No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35510.11631654872323671No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35290.11559591676831944No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC35280.11556316077036867No Hit
GTCCAAATATGGTCCCCCATGCCCATCATGCCCAGCACCTGAGTTCCTGG35060.1148425288154514No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC35020.11471150482364825No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC34580.11327024091381371No Hit
GCAGATATCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGCT34430.11277890094455195No Hit
GTAGTAAGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA34400.1126806329506996No Hit
GGACATAAGTAATTATTTAAATTGGTATCAGCAGATATCAGGGAAAGCCC33610.1100929091125876No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA33220.108815425192507No Hit
CATTAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATC32790.10740691728062327No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC32730.10721038129291854No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGG32130.10524502141587146No Hit
CCTCCACGCCATCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCCTGG31980.1047536814466097No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG31970.10472092544865891No Hit
GTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCCCCTGGCCGAAAGTG31880.10442612146710185No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC31840.1042950974752987No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA31670.10373824551013536No Hit
CCCCTAAACTCCTGATCTCTGCTGCATCCAGCTTGCAAAGTGGGGTCCCA31400.10285383356546417No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30930.1013143016617773No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCTACCTTGGTCC30660.1004298897171061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)