FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715842
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91900.3383849281364675No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTACCGCCTGG90440.3330090631192831No Hit
GTATCTACATATGAACAACCTGAAAACCGAGGACACAGCCATATACTACT80710.2971822366691435No Hit
CTCCTGGACAGTCGATCACCATCCCCTGCACTGGAACCAGAAGTGACGTT77140.28403714207232966No Hit
GTATGTACACGGGGTCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAG69530.25601636619508794No Hit
CCCTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTTGGTCAGCCCA68090.25071414316444035No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT67210.24747389575682238No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT63500.23381330725425115No Hit
GCCATATACTACTGTACCGCCGGGTGGGAGTATTATACAACTGGATATTT63410.2334819183148357No Hit
ATATACTACTGTACCGCCGGGTGGGAGTATTATACAACTGGATATTTTGA60550.2229511142400773No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCGGCCTCCGTGTCTGGCTCTC59330.21845895306133423No Hit
GTATATGGCTGTGTCCTCGGTTTTCAGGTTGTTCATATGTAGATACAGTG58110.21396679188259113No Hit
TATCAGCACTCTCCCTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCC55980.20612392031642487No Hit
GTATAATACTCCCACCCGGCGGTACAGTAGTATATGGCTGTGTCCTCGGT55760.2053138584645204No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT55350.20380419774051658No Hit
GTATTATACAACTGGATATTTTGACTACTGGGGACCGGGAAGCCTGGTCA54560.2008953392723141No Hit
TGTAGATACAGTGTGTTTTCTGAATCCCCTCTTGAGACGCTGAACCTGCC54080.19912793159543155No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA53910.198501974709869No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGCAGGGGCCACTGTCTTCTC53340.196403178093571No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC50030.1842154293217352No Hit
GGTTATAACTATGTCTCATGGTACCAACAACACCCAGGCAAAGCCCCCAA49700.18300033654387846No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA49580.18255848462465785No Hit
CTACTGTACCGCCGGGTGGGAGTATTATACAACTGGATATTTTGACTACT49050.18060697198143338No Hit
CCTATGAACATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA48250.1776612925199625No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA47580.17519428597098063No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT47370.17442104511234455No Hit
GTATAAGAGGTGCAAAAATAATCAGCCTCGTCCTCAGGCTGGAGGTCAGA46650.17176993359702075No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA45630.16801419228364536No Hit
GTCGATCACCATCCCCTGCACTGGAACCAGAAGTGACGTTGGTGGTTATA43970.1619019074010933No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA42570.15674696834351926No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41470.1526966590839968No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCG41110.15137110332633488No Hit
GTAGTATATGGCTGTGTCCTCGGTTTTCAGGTTGTTCATATGTAGATACA41040.1511133563734562No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC40820.1503032945215517No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG40370.14864634982447433No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG39610.145847954336077No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39260.14455921957168347No Hit
GTGCTGATACTTGTATAAGAGGTGCAAAAATAATCAGCCTCGTCCTCAGG38630.14223949699577515No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC38320.1410980462044552No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA37940.13969884846025651No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC37080.13653224303917533No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGT36240.1334392796046309No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC35430.13045677914989162No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG35240.12975718027779232No Hit
ACATAAGGGAGAGTGCTGATACTTGTATAAGAGGTGCAAAAATAATCAGC34660.1276215626682259No Hit
GGGTCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGC34520.12710606876246852No Hit
TCTCTGACCTCCAGCCTGAGGACGAGGCTGATTATTTTTGCACCTCTTAT34450.1268483218095898No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT33880.1247495251932918No Hit
GTACAGTAGTATATGGCTGTGTCCTCGGTTTTCAGGTTGTTCATATGTAG33830.12456542022694989No Hit
CTTATACAAGTATCAGCACTCTCCCTTATGTCTTCGGAAGTGGGACCAAG33550.12353443241543507No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA33550.12353443241543507No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT33550.12353443241543507No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTACCGCCTGGATGAGTTGGG33460.1232030434760196No Hit
CTGTATCTACATATGAACAACCTGAAAACCGAGGACACAGCCATATACTA33390.12294529652314089No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT33260.12246662361065187No Hit
TCCTTGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC32920.12121470983952674No Hit
ATCTACATATGAACAACCTGAAAACCGAGGACACAGCCATATACTACTGT32850.12095696288664806No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG32790.12073603692703773No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT32750.12058875295396418No Hit
TTATACAACTGGATATTTTGACTACTGGGGACCGGGAAGCCTGGTCACCG32740.1205519319606958No Hit
CATATGAACAACCTGAAAACCGAGGACACAGCCATATACTACTGTACCGC32380.1192263762030339No Hit
GACCAAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAAGGGAGAGTG32280.11885816627035004No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCT31790.11705393760019912No Hit
GCCTTGGGCTGACCAAGGACGGTGACCTTGGTCCCACTTCCGAAGACATA31150.11469739403102241No Hit
GTACCAACAACACCCAGGCAAAGCCCCCAAAGTCATGATTTATGATGTCA29920.11016841185901094No Hit
CTCTTATACAAGTATCAGCACTCTCCCTTATGTCTTCGGAAGTGGGACCA29760.10957927596671677No Hit
CATAAGGGAGAGTGCTGATACTTGTATAAGAGGTGCAAAAATAATCAGCC29760.10957927596671677No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28730.10578671366007301No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG28370.10446115790241112No Hit
CCTCTTATACAAGTATCAGCACTCTCCCTTATGTCTTCGGAAGTGGGACC28260.10405612697645887No Hit
CCCCTGCACTGGAACCAGAAGTGACGTTGGTGGTTATAACTATGTCTCAT27880.1026569292322602No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG27870.1026201082389918No Hit
GTTATAACCACCAACGTCACTTCTGGTTCCAGTGCAGGGGATGGTGATCG27660.1018468673803557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)