FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935944
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330591.7076423698206147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162590.839848673308732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156340.8075646816230221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG110910.5728988028579339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG94070.4859128156599571No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA89750.4635981206067944No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT73780.38110606505146843No Hit
GTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCACC68830.35553714363638617Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG67170.3469625154446616No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA67040.34629100841759886No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCG66780.34494799436347334TruSeq Adapter, Index 6 (96% over 33bp)
GTATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCC55580.28709508126268113TruSeq Adapter, Index 1 (96% over 32bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG53910.27846879868425944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG53330.2754728442558256No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA49300.2546561264168798No Hit
TATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGG48950.25284822288248004No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAA48630.25119528250817175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG46990.24272396308984145No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA46410.23972800866140756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT45300.23399437173802548No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA45280.23389106296463122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG44960.23223812259032284No Hit
TATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA44550.23012029273574028TruSeq Adapter, Index 16 (97% over 34bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT43970.2271243383073064No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGT43460.2244899645857525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA38490.19881773439727593No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAA36770.1899331798853686No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAGCAGAAGACGGCATA35480.18326976400143805No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35420.18295983768125526No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA35360.18264991136107242No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA35050.18104862537346122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT34960.180583735893187No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA34960.180583735893187No Hit
TATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCCG34760.17955064815924426TruSeq Adapter, Index 1 (96% over 33bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA34280.17707123759778176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT33660.17386866562255934No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTT32480.16777344799229732No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT31840.1644675672436806Illumina PCR Primer Index 7 (95% over 22bp)
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACACAAAAAAAAAAAA31290.16162657597533814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG31250.1614199584285496No Hit
GAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCA30620.15816573206663004Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA29790.15387841797076773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG28260.14597529680610596No Hit
GTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAA27620.14266941605748926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT26470.13672916158731865No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA25410.13125379659742223No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA25000.12913596674283967No Hit
GAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA24880.12851611410247404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT24320.12562346844743444No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTTCT23860.12324736665936617RNA PCR Primer, Index 7 (95% over 21bp)
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA23460.12118119119148074No Hit
GGTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCC23290.12030306661762943TruSeq Adapter, Index 6 (96% over 32bp)
GGTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATC22370.11555086304149294No Hit
GTATCAACGCAGAGTACGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA22240.11487935601443018TruSeq Adapter, Index 16 (97% over 37bp)
CTGGAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAG22190.1146210840809445Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAA22120.11425950337406454No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA21900.11312310686672755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT21860.11291648931993901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT20990.10842255767728819No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAGCAGAAGACGGCATAC20850.10769939626352827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20550.10614976466261422No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGATATCAAACGTAAGTACATCTG20240.10454847867500298No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA19440.10041612773923213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)