FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355800
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA151490.4514273794624233No Hit
GTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACAATTGGATCCG139580.4159365874009178No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT129620.38625663031169916No Hit
GTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGA125880.3751117468263901No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC109350.32585374575362064No Hit
ATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCC97810.29146552237916445No Hit
CAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGT95450.28443292210501225No Hit
GTATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTACTA89870.2678049943381608No Hit
GTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA72070.21476250074497885No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC71300.21246796590976819No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT69860.20817688777638715No Hit
GAGTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG67620.20150187734668334No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT67400.2008462959651946No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCT62270.18555933011502473No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61560.18344359020203826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG60870.18138744859645986No Hit
CTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT60550.18043387567793076No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT60330.179778294296442No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTG60070.17900351630013708No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC59560.1774837594612313No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA59380.17694737469455868No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATT56120.16723285058704332No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA56000.1668752607425949No Hit
CACCTGCACTGTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACA55690.16595148697776985No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA53900.1606174384647476No Hit
CTACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCT53210.1585612968591692No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC51790.15432981703319626No Hit
GTCCCAGGTGATGCTGCAGAGGTCGGGCCCAGGACTGGTGAAGCCTTCAC51740.15418082126467608No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC51170.1524822695035461No Hit
ATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACT50350.15003873889981525No Hit
GTACAAAATGTACCCAATCCACTCCAGGCCCCTCACTGGGTGCTGGCGGA49770.14831038798498122No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT49080.14625424637940282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48300.1439299123904881No Hit
GCGTAGTAGTAGTAAAGGGGATAATAACCACTAGTATCACCTCTGGCACA47380.1411883902497169No Hit
ATTTTGTACAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGT46990.14002622325525954No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA46920.13981762917933133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46860.1396388342571071No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45440.13540735443113416No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG44630.13299362298110734No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT43610.1299541093032958No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA42960.12801716431253352No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCGGCTACTTAGCCTGGTACC42810.12757017700697298No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC42730.12733178377734072No Hit
ATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGA42560.12682519816437213No Hit
GTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCA41810.12459026163656951No Hit
GTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT40660.12116335896060551No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA40200.11979259789021991No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT39960.11907741820132307No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT38610.11505453245127838No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGCT38530.11481613922164612No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGC38490.11469694260682996No Hit
ACCTGAGACAGTGCAGGTGAGGGACAGGGTCTGTGAAGGCTTCACCAGTC37370.11135943739197807No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG37130.11064425770308123No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC36810.10969068478455211No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC36730.10945229155491984No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA36310.10820072709935037No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT36080.10751534656415758No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG35850.10682996602896479No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG35500.10578699564932356No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCC35060.10447583288634603No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC34950.10414804219560164No Hit
ACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGC34420.10256868704928779No Hit
CCTCTGGCACAGTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAG34250.1020621014363192No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT34220.1019727039752071No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT34190.101883306514095No Hit
TCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTGGC33640.10024435306037309No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)