FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333149
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT51870.22231756308748393No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT35770.15331211165682088RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35060.15026901410925747No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT25870.11088018810628897RNA PCR Primer, Index 17 (95% over 23bp)
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC23770.10187947705011552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)