FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542553
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT58820.23134227683749364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52630.20699666830937252No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT30110.11842427670140997RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.10595649333563549No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT26410.10387197435019055No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)