FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003301
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGCTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGG96690.48265338059532736No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC69460.34672772588842116No Hit
CTACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG56020.2796384567271718No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCGAAAGCCAGTG52070.2599210003888582No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC51720.2581738840044507No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT46830.23376417223372822No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA46630.23276582001406676No Hit
GGGTTAACAATGATGGCAGCCACTACAAGGGGGACGGGATCCCTGATCGC46430.23176746779440532No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA45000.22462924942382598No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA44700.2231317210943338No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44400.22163419276484161No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41810.2087055315202259No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCG41470.2070083327468014No Hit
TTGTTAACCCTCATCAAATAGCGAGGGCCCTTCTCTGGCTGCTGCTGAAG41460.2069584151358183No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC40040.19987011437622204No Hit
GTATCAACGCAGAGTACGGGACTCAAAAACCACACCCCTCCTTGGGAGAA38190.19063535634435363No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37650.18793980535126772No Hit
ACTCTGGACAGTGGTCACAGCACCTACGCCATCGCATGGCTTCAGCAGCA36670.18304787947492662No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG36040.17990306998299307No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35860.17900455298529774No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA35460.17700784854597487No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG35390.17665842526909337No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA34420.17181641700373534No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33980.16962004212048015No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC33680.16812251379098797No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG32090.16018561364467945No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG31620.15783948592847505No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA31550.15749006265159354No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCATCGTCTCCTCAGCTTCC31340.15644179282094903No Hit
GGGTGGATCAGCGCTTACAATGGCGACACAAGTTATTCAGAAAAATTCCA29650.1480057165648098No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29390.1467078586792499No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA29360.1465581058463007No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTG29110.14531016557172388No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT29060.1450605775168085No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28920.1443617309630455No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG28230.1409174158052135No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT28190.1407177453612812No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT27110.13532664337510938No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26930.13442812637741408No Hit
GAAGTACACGGCCGTGTCGTCTGATCTCAGGCGCCTCAGCTCCATGTAGG26910.13432829115544795No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC26800.13377919743463412No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCGAAAGCCAGTGT26680.13318018610283727No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGCGCTTACAATGGC26630.1329305980479219No Hit
CACCTGCACTCTGGACAGTGGTCACAGCACCTACGCCATCGCATGGCTTC26550.13253125716005731No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT26340.1314829873294128No Hit
TTCCAGGACAGAGTCATCCTGACCACAGAGAGATCCACTAAAACAGCCTA26130.13043471749876828No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT25020.12489386267964724No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC24910.12434476895883345No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG24870.12414509851490114No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24620.12289715824032434No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAACTTATCTTG24430.12194872363164597No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23870.11915333741659391No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23660.1181050675859494No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAACTTATCTTGACTCAACCGC23650.11805514997496633No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG23280.11620819836859264No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG23000.1148105052610666No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCC22780.11371231781943901No Hit
CTATTTGATGAGGGTTAACAATGATGGCAGCCACTACAAGGGGGACGGGA22750.1135625649864898No Hit
TATCAACGCAGAGTACGGGACTCAAAAACCACACCCCTCCTTGGGAGAAT22730.11346272976452366No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA22720.11341281215354057No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG22650.11306338887665908No Hit
GTCTGGCGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA22490.11226470710092992No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT22150.11056750832750546No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCC22010.10986866177374244No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG21900.10931956805292865No Hit
GTGTACTTCTGTGCGAGAGATGAGACGTTCGATGTGCTCGACGGTATGGA21820.10892022716506407No Hit
GGTTACAGGTTCAGCACGTACGGTATAAGCTGGGTGCGGCAGGCCCCTGG21730.1084709686662164No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21570.10767228689048726No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA21500.10732286361360575No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA21490.10727294600262266No Hit
CTCCCAGGTTCAGTTGGTGCAGTCTGGCGCTGAGGTGAAGAAGCCTGGGG21090.10527624156329977No Hit
CGTCCATACCGTCGAGCACATCGAACGTCTCATCTCTCGCACAGAAGTAC21050.10507657111936748No Hit
ACCTACGCCATCGCATGGCTTCAGCAGCAGCCAGAGAAGGGCCCTCGCTA20850.10407821889970603No Hit
GAGTCATCCTGACCACAGAGAGATCCACTAAAACAGCCTACATGGAGCTG20830.1039783836777399No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT20720.10342928995692609No Hit
CCCTTGTAGTGGCTGCCATCATTGTTAACCCTCATCAAATAGCGAGGGCC20330.10148250312858625No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC20110.10038431568695867No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG20060.10013472763204331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)