FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005112444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003301
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGCTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGG81110.40488174268370053No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC67730.33809197918834966No Hit
CTACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG57010.284580300214496No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC51790.25852330728133216No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCGAAAGCCAGTG45700.22812348219264106No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45020.2247290846457921No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41410.20670882708090296No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT40540.20236599492537566No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39880.1990714326004929No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC38650.19293156644957496No Hit
GGGTTAACAATGATGGCAGCCACTACAAGGGGGACGGGATCCCTGATCGC38600.19268197839465961No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT38220.19078510917730285No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCG37460.18699137074258937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37130.18534408958014797No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA35570.17755694226678867No Hit
TTGTTAACCCTCATCAAATAGCGAGGGCCCTTCTCTGGCTGCTGCTGAAG34220.17081806478407388No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAACTTATCTTGACTCAACCGC33390.16667490307247887No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT32550.1624818237499008No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32210.16078462497647633No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31290.15619220476603368No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA31210.1557928638781691No Hit
ACTCTGGACAGTGGTCACAGCACCTACGCCATCGCATGGCTTCAGCAGCA30820.15384607704982925No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG29940.1494533272833189No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA29420.14685761151219912No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA29160.14555975362663923No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA28680.14316370829945177No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCATCGTCTCCTCAGCTTCC28680.14316370829945177No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA28340.1414665095260273No Hit
GTATCAACGCAGAGTACGGGACTCAAAAACCACACCCCTCCTTGGGAGAA28290.14121692147111192No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC28090.1402185692514505No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG27560.13757293586934763No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTG27450.13702384214853383No Hit
GAAGTACACGGCCGTGTCGTCTGATCTCAGGCGCCTCAGCTCCATGTAGG27300.13627507798378777No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26690.13323010371382035No Hit
GCACAGGGTCTCTCTCCCAACTTATCTTGACTCAACCGCCCTCTGCCTCT26620.13288068043693882No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA25450.12704031995191936No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG24890.1242449337368673No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAACTTATCTTG24680.12319666390622278No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG24620.12289715824032434No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT24310.1213497122998491No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGCGCTTACAATGGC24220.12090045380100144No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24070.12015168963625536No Hit
CACCTGCACTCTGGACAGTGGTCACAGCACCTACGCCATCGCATGGCTTC23480.11720655058825409No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG23180.11570902225876191No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC22910.11436124676221897No Hit
GGGTGGATCAGCGCTTACAATGGCGACACAAGTTATTCAGAAAAATTCCA22910.11436124676221897No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22630.11296355365469292No Hit
GCTTATACCGTACGTGCTGAACCTGTAACCAGAGGCCTTGCAGGAGACCT22600.1128138008217437No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCGAAAGCCAGTGT22520.11241445993387914No Hit
CCACTGCACAGGGTCTCTCTCCCAACTTATCTTGACTCAACCGCCCTCTG22280.11121643727028539No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG22230.11096684921537002No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG22190.11076717877143774No Hit
TTCCAGGACAGAGTCATCCTGACCACAGAGAGATCCACTAAAACAGCCTA22030.10996849699570858No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCC21870.10916981521997943No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21830.10897014477604713No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG21170.10567558245116436No Hit
CCCTTGTAGTGGCTGCCATCATTGTTAACCCTCATCAAATAGCGAGGGCC21140.10552582961821513No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT21120.10542599439624899No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC20940.10452747739855368No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG20550.10258069057021386No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT20440.10203159684940007No Hit
CTATTTGATGAGGGTTAACAATGATGGCAGCCACTACAAGGGGGACGGGA20390.1017820087944847No Hit
GTCTGGCGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA20240.10103324462973863No Hit
CTCCCAGGTTCAGTTGGTGCAGTCTGGCGCTGAGGTGAAGAAGCCTGGGG20200.10083357418580632No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT20070.10018464524302638No Hit
AGCTTATACCGTACGTGCTGAACCTGTAACCAGAGGCCTTGCAGGAGACC20040.10003489241007717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)