FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670613
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT59720.35747357407131397No Hit
GTATTACTGTCAGCAGTATGCTAACTCACCGTACACTTTTGGCCAGGGGA55910.3346675741180034No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55860.33436828278003344No Hit
GTCTGGGACAGCCTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT36200.21668692869024725No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT33150.19843015707408002No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32900.19693370038423022No Hit
CTCCTGTAGGGCCAGTCAGAGTCTCAGCAGTAGCTACATAGCCTGGTACC31750.19004999961092126No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31730.18993028307573329No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31290.18729651930159769No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC30710.18382473978114622No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT30070.1799938106551308No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28120.16832144847430253No Hit
GTGTACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTTTATTA25320.1515611335479851No Hit
GTAATAAACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTACA23220.13899089735324696No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23140.13851203121249506No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22500.13468110208647963No Hit
CCTGTAGGGCCAGTCAGAGTCTCAGCAGTAGCTACATAGCCTGGTACCAG22070.13210719657993802No Hit
GTGTATTACTGTCAGCAGTATGCTAACTCACCGTACACTTTTGGCCAGGG21850.13079031469287022No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA20720.12402633045474924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20590.12324817297602736No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCCATGG20210.12097355880745571No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19340.1157658895267785No Hit
GTTTATTACTGTGCGCGAGGGGCCGGCGTTGGAACTACGGTTGTTGAGGC19140.11456872417489868No Hit
GCCTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA19070.11414971630174073No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA18850.11283283441467294No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18260.10930119662662748No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA18230.1091216218238455No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18110.10840332261271761No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18100.10834346434512362No Hit
GTATGCTAACTCACCGTACACTTTTGGCCAGGGGACCAAGTTGGAGATCA17910.10720615726083778No Hit
TTATTACTGTGCGCGAGGGGCCGGCGTTGGAACTACGGTTGTTGAGGCAT17600.10535055096542406No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT17280.10343508640241635No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17150.10265692892369446No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16910.1012203305014387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)