FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005103
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACAATTGGATCCG93960.46860435598570244No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA93230.4649636452591213No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT87960.4386807061781864No Hit
GTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGA86240.430102593233365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78230.390154520740331No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68250.3403815165604959No Hit
ATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCC66110.32970874812914847No Hit
CAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGT61940.3089118115129248No Hit
GTATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTACTA60160.3000344620700283No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT49680.2477678204062335No Hit
GTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA47250.23564874223418944No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45660.2277189750351977No Hit
GAGTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG42930.21410371437277786No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC42110.21001414889908399No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT41440.206672674670578No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39870.1988426529709446No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39270.19585028799019305No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCT38130.19016479452676496No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA37460.186823320298259No Hit
CACCTGCACTGTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACA37350.18627472005178786No Hit
CTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT36820.1836314643187906No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35860.17884368034958803No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTG35850.17879380759990882No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35010.1746044966268566No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC34720.17315818688615997No Hit
ATTTTGTACAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGT34650.1728090776384056No Hit
ATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACT34630.1727093321390472No Hit
CTACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCT34620.17265945938936803No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATT34450.17181162264482175No Hit
GCGTAGTAGTAGTAAAGGGGATAATAACCACTAGTATCACCTCTGGCACA33470.16692409317626075No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA32340.1612884724625119No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA32160.16039076296828642No Hit
GTCCCAGGTGATGCTGCAGAGGTCGGGCCCAGGACTGGTGAAGCCTTCAC31870.1589444532275898No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31680.15799687098368514No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT31320.15620145199523416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA30120.15021672203373093No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30110.15016684928405175No Hit
GTACAAAATGTACCCAATCCACTCCAGGCCCCTCACTGGGTGCTGGCGGA29560.1474238480516961No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28560.14243657308377675No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC28420.14173835458826803No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28120.14024217209789222No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT27340.13635209762291514No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT27120.13525489712997288No Hit
ATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGA26920.134257442136389No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT26610.132711386896334No Hit
GTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCA26470.1320131684008253No Hit
GTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT25310.12622792943803884No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA25310.12622792943803884No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCGGCTACTTAGCCTGGTACC25260.12597856568964289No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC24010.1197444719797437No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23980.11959485373070611No Hit
GTGTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGG23330.11635312500155853No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23140.11540554275765384No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG22440.1119144502801103No Hit
CATTTTGTACAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAG22220.11081724978716805No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG22210.11076737703748885No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG22140.1104182677897345No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC22110.11026864954069693No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA22100.11021877679101771No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22030.10986966754326336No Hit
ACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGC21980.1096203037948674No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCC21750.10847323055224595No Hit
TCACAGACCCTGTCCCTCACCTGCACTGTCTCAGGTGGCTCCATCAACAG21460.10702692081154934No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG21370.1065780660644366No Hit
TCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTGGC21120.10533124732245674No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21080.10513175632373999No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGC21080.10513175632373999No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCA20740.10343608283464739No Hit
CCTCTGGCACAGTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAG20710.1032864645856098No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGCT20670.10308697358689305No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC20460.10203964584362998No Hit
ATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAA20360.10154091834683804No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20320.10134142734812128No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC20200.10074295435197095No Hit
GCCGTATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTA20090.10019435410549983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)