FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005112569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52804219
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA238150.029.973691
TCGGAAG254800.027.9359552
CGGAAGA272350.026.3753223
TATGCCG106950.014.19476645-49
AGAGCAC574500.012.8695967
CTCGTAT122700.012.41947540-44
AGCACAC611100.012.3123069
CGTATGC122500.012.2508645-49
GAGCACA636700.011.8741958
TCTCGTA119600.011.46845840-44
GAAGAGC656800.011.4225435
ATGCCGT136500.011.06871945-49
TCGTATG139050.011.01128440-44
TGCCGTC140450.010.69548145-49
GCCGTCT145100.09.94312150-54
ATCTCGT149600.09.1589440-44
CGTCTTC180700.08.45764750-54
AAGAGCA928400.08.4166496
CCGTCTT182850.08.2154650-54
CGTCTGA204100.07.49389415-19
Produced by FastQC (version 0.11.5)