FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005112574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44081317
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTGATTGGTGTAGATCTC527080.11956993027227385Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG203950.039.6728329
GAGCGTC250650.032.4835748
AGAGCGT280300.029.1504787
ATCGGAA287450.028.4397181
TCGGAAG298100.027.509862
CGGAAGA322900.025.5288283
AAGAGCG331850.024.9501466
CGCCGTA99650.017.05114755-59
TCGCCGT112300.015.13042755-59
GCCGTAT120700.014.1615255-59
GTCGCCG122950.014.02027955-59
GAAGAGC603150.013.9433835
TGGTCGC145050.012.004271550-54
CCGTATC137350.011.96982255-59
CGTATCA144950.011.77277160-64
GTGGTCG151050.011.60422350-54
GGTCGCC148100.011.42421350-54
TCTCGGT186700.09.60568945-49
GGAAGAG928450.09.4407974
TCGGTGG189450.09.10658545-49
Produced by FastQC (version 0.11.5)