FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005112590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40439489
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG281000.016.3595899
GAGCGTC327800.014.1340398
AGAGCGT363700.012.8985457
CGCCGTA85400.012.563189555-59
ATCGGAA398050.011.8936511
TCGGAAG407900.011.626542
AAGAGCG421050.011.3309856
CGGAAGA422850.011.1800493
TCGCCGT102350.010.59594755-59
GCCGTAT106300.010.29768855-59
GTCGCCG115850.09.33617155-59
CGTATCA126500.08.71012660-64
TGGTCGC131250.08.48365150-54
CCGTATC125750.07.724900255-59
GAAGAGC640600.07.7193735
GTGGTCG144200.07.711715750-54
GGTCGCC136350.07.166805750-54
TCTCGGT180050.06.38552145-49
CTCGGTG200050.05.87033345-49
CGGTGGT202750.05.79937150-54
Produced by FastQC (version 0.11.5)