FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005112608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27338591
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGGACGTGTGTAGATCTC394970.1444734295194657Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG204050.028.1445319
GAGCGTC232900.024.876088
AGAGCGT251400.023.1318177
ATCGGAA273550.021.2765351
TCGGAAG279800.020.873042
AAGAGCG287800.020.3070356
CGGAAGA290000.020.2021753
CGCCGTA88350.019.29736955-59
TCGCCGT97350.017.64736255-59
GCCGTAT103450.016.55071655-59
GTCGCCG105150.016.20040955-59
CGTATCA115700.014.86091360-64
TGGTCGC117400.014.74526150-54
GTGGTCG123100.014.14494350-54
GAAGAGC466200.012.909465
TTGGACG138550.012.56896330-34
GATCGGA226350.012.0832981
TCTCGGT147350.012.03331945-49
GACGTGT148750.011.62793535-39
CTCGGTG158450.011.2909945-49
Produced by FastQC (version 0.11.5)