FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005112625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52410975
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCGATAATCTCGTATG555380.10596635532920348TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA368100.026.3519731
TCGGAAG379850.025.608972
CGGAAGA396750.024.53643
TATGCCG145950.016.91889245-49
CGACCGA150350.016.30597130-34
ACGACCG155650.015.67622630-34
CGTATGC160300.015.35004645-49
GACCGAT161650.015.26477330-34
CTCGTAT162400.015.21338840-44
AGAGCAC672600.014.9046447
CCGATAA166750.014.6850435-39
TCTCGTA156050.014.5688340-44
AGCACAC710350.014.3268599
ATGCCGT174150.014.13759745-49
GAGCACA726900.013.9807248
GAAGAGC755600.013.2770165
GCCGTCT184450.012.92375750-54
ACCGATA164150.012.20600930-34
ATCTCGT190000.012.07244740-44
GTCACGA210000.011.95725725-29
Produced by FastQC (version 0.11.5)