FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3365739
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG122120.36283264982816554No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA99580.2958637018497275No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG92640.27524415886080295No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTTCCAATTATGTCTTCGGAAC90720.26953961670824744No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTG87830.26095309232236963No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT85520.2540898150450763No Hit
GTAATACACGGCCGTGTCCGCTGCGGTCACAGAGTTCAGCTTCAGGGAGA85200.25313905801965036No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT84600.25135638859697673No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC81640.24256188611178703No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT79500.2362036985042512No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC77180.22931071006991335No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG73490.21834729312047071No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC73020.2169508687393764No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC72500.21540588857305928No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA71030.21103834848750896No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA70580.20970134642050378No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA64980.19306309847555025No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGGAACTAC62390.18536790880100923No Hit
CTCCTGCACTGGAACCAGCAGTGATGTGGGGAGTTATAACCTTGTCTCCT61760.18349610590720197No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATTGGAACTA60880.18088152408728067No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG59330.1762762947453739No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC58830.17479073689314592No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA58740.17452333647974486No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC58620.17416680259521014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57880.1719681769739127No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG57530.17092828647735311No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT55630.16528316663888673No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG54270.1612424492808266No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT53610.15928151291588563No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG53270.1582713335763706No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG50450.1498927872898047No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT49080.14582235877469998No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC49000.1455846695183435No Hit
GTCTCTGGTGGCTCCATGCGTAATTACTATTGGAGCTGGATTCGGCAGCC48460.1439802670379373No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT47450.14097944017643674No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT46300.13756265711631235No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC45530.13527489802388123No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44060.13090735793833094No Hit
GTAATAGATATACCCAATCCATTCCAGTCCCTTCCCTGGGGGCTGCCGAA43800.13013486785517236No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC43720.1298971785988159No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT42680.12680721826618166No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTGGGGAGTTATA41840.12431148107443864No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT41680.12383610256172567No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC41390.12297447900743344No Hit
GTGTATTACTGTGCGAGGGATCTAAGGTCGCCCGTGGGCGAGTTTGACTA40460.12021134140228935No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG40190.11940914016208624No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC40100.1191417397486852No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT39660.11783444883872458No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC39390.11703224759852145No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG39290.11673513602807586No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCC39120.11623004635831834No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAG39080.1161112017301401No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA38790.11524957817584786No Hit
TATGCAGGTAGTTCCAATTATGTCTTCGGAACTGGGACCAAGGTCACCGT38310.11382344263770898No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG37970.11281326329819394No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG37560.11159510585936698No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG37450.11126828313187684No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCT37150.11037694842054002No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT37040.11005012569304988No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCT36690.1090102351964903No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT36160.1074355438731286No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC35650.10592027486385604No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG34790.10336511535802391No Hit
GCTCATATGCAGGTAGTTCCAATTATGTCTTCGGAACTGGGACCAAGGTC33930.10080995585219174No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)