FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920061
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137011.4891403939521402No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT75820.8240757949744637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66570.7235389827413616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63760.6929975295116302No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT60310.6555000157598246RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50010.5435509167326948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG43890.4770335879903615No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA43210.46964277368565777No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT37730.4100815054653985No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTT37060.4027993796063522No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT35220.3828007056053892RNA PCR Primer, Index 29 (96% over 25bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA32470.3529113830496021No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCG32180.34975941812553735TruSeq Adapter, Index 6 (96% over 33bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24990.2716124256978613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG23990.2607435811321206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG23910.2598740735668613No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTTCT23830.25900456600160204Illumina PCR Primer Index 12 (95% over 21bp)
TATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA22070.23987539956589837TruSeq Adapter, Index 16 (97% over 34bp)
GTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCACC19980.21715951442350018Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCC19870.2159639415212687TruSeq Adapter, Index 1 (96% over 32bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19780.21498574551035202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG19110.2077036196513057No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA19010.20661673519473167No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT18840.20476903161855572No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG18790.2042255893902687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA18540.2015083782488335No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA18190.19770428265082426No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCT17600.19129166435703718RNA PCR Primer, Index 29 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT17600.19129166435703718No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAA15930.17314069393225015No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA15350.1668367640841205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG14870.16161971869256495No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA14740.16020676889901864No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG14720.15998939200770385RNA PCR Primer, Index 29 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT14540.1580329999858705No Hit
TATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGG14300.15542447729009273No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA14030.15248988925734272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG13700.14890317055064828No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA13570.14749022075710197No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13470.1464033363005279No Hit
TATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCCG13130.14270792914817607TruSeq Adapter, Index 1 (96% over 33bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT13020.14151235624594458No Hit
GTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAA11680.12694810452785193No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA11200.1217310591362964No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTTCTGCTTG10890.11836171732091676Illumina PCR Primer Index 12 (96% over 26bp)
GAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCA10820.1176008982013149Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAA10820.1176008982013149No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10770.11705745597302789No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTTCTGCT10340.11238385280975935Illumina PCR Primer Index 12 (95% over 24bp)
GTATCAACGCAGAGTACGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA10170.11053614923358342TruSeq Adapter, Index 16 (97% over 37bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT9870.1072754958638612No Hit
GGTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCC9860.1071668074182038TruSeq Adapter, Index 6 (96% over 32bp)
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA9740.10586254607031491No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACACAAAAAAAAAAAA9710.10553648073334267No Hit
GAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA9250.10053681223310194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)