FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3523946
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT324900.9219778055622873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC262860.7459251645740315No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC149390.42392817597091437No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT146790.4165500833440694No Hit
GACTTACAGTTCCCCTACATTCACTTTCGGCCCTGGGACCACAGTGGATA145850.41388261908667157No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG143620.40755448579518533No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG138690.39356448708351377No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG127630.3621792161400884No Hit
CCCCTAAACTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCA120470.34186108413693056No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC117800.3342843505547474No Hit
ATTTAAATTGGTATCAGCAAAAACCAGGGAATGCCCCTAAACTCCTGATC117530.33351816401272893No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT115970.32909130843662193No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC113010.3206916337537522No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC110800.3144202550209339No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA109040.3094258538581465No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG107110.3039490389466808No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATGACTT105400.2990965241805635No Hit
GTCGGAGACAGAGTCACCATGACTTGCCGGGCAAGTCAGAGCATTTCCAT103430.29350620015176165No Hit
CTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT100400.28490788451355387No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT98120.27843786482539745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA97860.27770005556271293No Hit
ACTTACTACTGTCAACAGACTTACAGTTCCCCTACATTCACTTTCGGCCC97090.27551500505399346No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG94980.26952739911451534No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA93290.2647316389070661No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGGAGACT92570.2626884747950167No Hit
GTACAGTGGAAGGTGGATAACGTCCTCCAATCGGGTAACTCCCAGGAGAG92480.26243307928101056No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG87500.24830119417266894No Hit
GATTTCACTCTCACCATCAGCAGTCTCCAACCTGAAGATTTTGCAACTTA87120.2472228575579762No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG86580.24569048447393915No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG82310.2335733861983129No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC81740.23195588127627378No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC80460.22832358952151935No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAAAGTG79630.22596827533679573No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATGACTTGCCGGGCAAGTCAGA78140.22174006071602687No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT78070.22154141976068872No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCC77750.2206333468220001No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG74990.2128012177258108No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTCCAACCTGAAGATT74870.21246069037380255No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA74580.21163774927311596No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA71450.20275566084156796No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGGAGACTGCTGATGGTGAGAGTGA70820.2009678922435247No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGC69600.19750586416477436No Hit
GTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC68720.19500866358338068No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC68610.19469651351070646No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG68270.1937316860133498No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA67210.19072369440394377No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG67050.19026965793459946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66600.1889926803645686No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA66340.18825487110188407No Hit
CCATAGATCAGGAGTTTAGGGGCATTCCCTGGTTTTTGCTGATACCAATT65410.18561578412382027No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA65370.1855022750064842No Hit
AGTCAGAGCATTTCCATCTATTTAAATTGGTATCAGCAAAAACCAGGGAA65030.18453744750912754No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG64930.18425367471578735No Hit
CACCATCAGCAGTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAAC63410.17994032825701642No Hit
TTACTACTGTCAACAGACTTACAGTTCCCCTACATTCACTTTCGGCCCTG62100.1762229046642599No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC61910.17568373635691353No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCC61220.1737257040828662No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT60400.17139876717747662No Hit
ACATTCACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGC60240.1709447307081323No Hit
GTGGAAGGTGGATAACGTCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA59700.16941235762409526No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCT59190.16796511637806028No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC57350.16274369698060073No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT57310.16263018786326464No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGAC57280.1625450560252626No Hit
GTTCAGTGCCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTC56990.16172211492457603No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT56920.16152347396923789No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC56830.16126807845523172No Hit
GATATCCACTGTGGTCCCAGGGCCGAAAGTGAATGTAGGGGAACTGTAAG56570.16053026919254723No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC56060.15908302794651225No Hit
ACTGTCAACAGACTTACAGTTCCCCTACATTCACTTTCGGCCCTGGGACC55930.15871412331516999No Hit
GTTTAGGGGCATTCCCTGGTTTTTGCTGATACCAATTTAAATAGATGGAA55320.1569831092757948No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC55180.15658582736511853No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTC54640.1550534542810815No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA54450.15451428597373512No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGTTGACCCAGTC53890.15292515833103004No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGA53660.1522724809063476No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA53400.1515346716436631No Hit
GTGCCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTCCAA53400.1515346716436631No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA53100.15068335326364252No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT52880.1500590531182941No Hit
CACCATGACTTGCCGGGCAAGTCAGAGCATTTCCATCTATTTAAATTGGT52630.14934962113494363No Hit
CCCCTACATTCACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACT51330.14566057482152112No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG51280.14551868842485102No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC50990.14469574732416446No Hit
GTCTCCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGT50750.144014692620148No Hit
CATCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCACTCTCACCATC50620.14364578798880573No Hit
GTGGGGTCCCATCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCACT50500.1433052606367975No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA50350.1428796014467872No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC49970.1418012648320945No Hit
CCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATGACTTGCCG49780.1412620965247481No Hit
GTGGATAACGTCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA49740.141148587407412No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT49280.13984323255804715No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT48900.13876489594335442No Hit
TCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGCC48200.13677848638997306No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG47960.1360974316859566No Hit
GTCAACAGACTTACAGTTCCCCTACATTCACTTTCGGCCCTGGGACCACA47540.1349055859539278No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG46850.13294755367988045No Hit
TCAAGGTTCAGTGCCAGTGGATCTGGGACAGATTTCACTCTCACCATCAG46060.13070574861249293No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT46040.1306489940538249No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC45440.12894635729378373No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG44750.1269883250197364No Hit
GCAAAAACCAGGGAATGCCCCTAAACTCCTGATCTATGGTGCATCCAGTT44290.12568297017037153No Hit
GTCCCAGATCCACTGGCACTGAACCTTGATGGGACCCCACTTTGCAAACT43660.12389520157232829No Hit
GTTCCCCTACATTCACTTTCGGCCCTGGGACCACAGTGGATATCAAACGA43400.1231573923096438No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT43230.12267497856096546No Hit
CCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTTTG43090.12227769665028919No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA42900.12173852834294283No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC42830.1215398873876047No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG42810.12148313282893666No Hit
GATCAGGAGTTTAGGGGCATTCCCTGGTTTTTGCTGATACCAATTTAAAT42540.12071694628691812No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA42330.12012102342090372No Hit
GTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGCCAGTGGA41980.11912781864421304No Hit
ATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGCCAGT41730.11841838666086257No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT41370.11739680460483787No Hit
GAGTGAAATCTGTCCCAGATCCACTGGCACTGAACCTTGATGGGACCCCA40480.11487122674411014No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC40360.11453069939210193No Hit
CTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCA40060.11367938101208135No Hit
CTGTGGTCCCAGGGCCGAAAGTGAATGTAGGGGAACTGTAAGTCTGTTGA40050.11365100373274732No Hit
CCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTC39950.11336723093940713No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG39560.11226051704538037No Hit
AGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGC39510.11211863064871028No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA39340.11163621690003195No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC38880.11033086205066707No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT38810.11013222109532891No Hit
GAGCATTTCCATCTATTTAAATTGGTATCAGCAAAAACCAGGGAATGCCC38800.1101038438159949No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCA38310.10871335712862797No Hit
ATGTAGGGGAACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCA38160.10828769793861767No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT37490.10638642022323837No Hit
GTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAAAGTGAATGTAGGGGA36660.10403110603851477No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT36640.10397435147984674No Hit
CAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATG36110.10247035567514372No Hit
GCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCT35920.10193118736779734No Hit
ACGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35820.10164741457445717No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCACCATAGATCAGGAGT35400.10045556884242833No Hit
ACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGA35250.10002990965241806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)