FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879660
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGATACGACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT180770.62774772021697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC166920.5796517644444136No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT157670.5475299167262802No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA112980.3923379843453741No Hit
GTATATTACTGTGCGAAATGCGAATATGGTTCAGGGAATTCCTATTACTA89410.3104880437273845No Hit
TTATTACTGCATGCAACCTCTACAAACCTTCGGCCAAGGGACACGACTGG84270.29263871429265953No Hit
CTACAAACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGC82680.28711722911732634No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC82460.2863532500364626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG82230.28555454463374147No Hit
AGTATGTACTACGCAGACTCCGTGAAGGGCCGGTTCGCCATCTCCAGAGA78890.273955953133356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC76650.2661772570372891No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT72790.2527728968003167No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA70530.24492474806053494No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65240.22655452379794835No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA63870.22179701770347887No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG63540.22065104908218333No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC62210.2160324482751436No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA60130.2088093733287958No Hit
GTACTACGCAGACTCCGTGAAGGGCCGGTTCGCCATCTCCAGAGACAATT59800.2076634047075002No Hit
GCATAGTAATGGATACGACTATTTGGATTGGTACCTGCAGAAGCCAGGGC58320.20252390907259887No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG56350.19568282366668288No Hit
GTTTATTACTGCATGCAACCTCTACAAACCTTCGGCCAAGGGACACGACT55770.19366869699895128No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT54730.19005715952577734No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC53950.1873485064208969No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA53760.18668870630560552No Hit
GGTTTGTAGAGGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCA53120.18446622170672927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG52040.1807157789461256No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT51040.17724314676038141No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC50760.17627080974837306No Hit
GGATACGACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA50490.17533319905822217No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT50160.17418723043692658No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC49470.17179111422876311No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC49360.17140912468833128No Hit
TTTTAAAAGGTGTCCAGTGTCAGGTGCAGTTGTTGGAGTCCGGGGGAGGC49060.170367335032608No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48690.16908246112388267No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT48100.16703360813429363No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACGACTATTTGGATTGG48050.16685997652500642No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGAATATTGTGATGACTCAGT46310.1608175965218116No Hit
TGGTAATAGTATGTACTACGCAGACTCCGTGAAGGGCCGGTTCGCCATCT45640.15849093295736302No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA45580.15828257502621837No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT44530.154636311231187No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT43570.15130258433287264No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC43220.15008716306786218No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC43070.14956626824000055No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA42670.14817721536570289No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA42560.14779522582527105No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA42200.14654507823840313No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTT42020.1459200044449692No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC41870.14539910961710759No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG41620.14453095157067153No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA41270.14331553030566108No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG41100.14272518283408459No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG40230.13970399283248716No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG40110.13928727697019785No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA39260.13633553961231534No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG39240.13626608696860046No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT39110.13581464478445374No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG38960.1352937499565921No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA38890.13505066570359No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT38540.13383524443857955No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA38510.13373106547300723No Hit
GTACAGTGGAAGGTGGATAACGTCCTCCAATCGGGTAACTCCCAGGAGAG38440.13348798122000513No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT38280.13293236007028608No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA37990.13192529673642028No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAGCG37950.13178639144899051No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG37500.13022370696540564No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCT37220.12925136995339728No Hit
GTACATACTATTACCACTACCATTAATAGTTGAGACCCACTCCAGCCCCT37120.12890410673482286No Hit
CTATTACCACTACCATTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGG36760.12765395914795497No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACGACTATTTGGATTGGTACCT36160.12557037983650848No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35980.12494530604307452No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTG35630.12372988477806408No Hit
TTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC35530.12338262155948967No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC35000.12154212650104526No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG34830.12095177902946876No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCT33890.11768750477486925No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA33780.1173055152344374No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT33380.11591646236013974No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGA33350.11581228339456742No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC33300.1156386517852802No Hit
CTCCTGCATAGTAATGGATACGACTATTTGGATTGGTACCTGCAGAAGCC33160.11515248327927602No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA33040.11473576741698671No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC32690.11352034615197627No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC32660.11341616718640395No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG32660.11341616718640395No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC32480.11279109339297No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA32390.11247855649625302No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC32240.11195766166839141No Hit
CTATTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCT32090.11143676684052979No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT31270.10858920844821958No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA30950.10747796614878145No Hit
ATATTACTGTGCGAAATGCGAATATGGTTCAGGGAATTCCTATTACTACT30940.107443239826924No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATC30940.107443239826924No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30850.10713070293020704No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30740.10674871338977518No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG30510.10595000798705402No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA30040.10431787085975427No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGT29990.10414423925046708No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29950.10400533396303731No Hit
GGACTGAACAGAGAGAACTTACCATGGAATTTGGGCTGAGCTGGCTTTTT29760.10334553384774592No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC29670.10303299695102895No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG29370.1019912072953057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)