FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971190
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGA107470.5452036587036257No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT100660.510655999675323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC98260.49848061323363047No Hit
GTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACAATTGGATCCG82320.4176157549500555No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA71830.36439917004449085No Hit
GTATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTACTA64320.32630035663736123No Hit
ATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCC60670.3077836230906204No Hit
CAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGT59890.3038266224970703No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC51550.2615171546121886No Hit
GTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA51480.26116203917430586No Hit
GAGTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG50950.25847330800176543No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT48550.24629792156007282No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT47400.24046388222342846No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45380.23021626530167058No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCT45110.22884653432698016No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42780.2170262633231703No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42370.21494630147271443No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTG42070.21342437816750287No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40580.2058654924182854No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC40360.20474941532779692No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40340.20464795377411615No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA40020.20302456891522377No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39930.20256799192366035No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATT39080.19825587589222754No Hit
CTACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCT36820.18679072032630034No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36680.18608048945053496No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT35650.1808552194359752No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35050.17781137282555207No Hit
CTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT33880.17187587193522696No Hit
ATTTTGTACAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGT33010.16746229435011337No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32840.16659987114382682No Hit
CACCTGCACTGTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACA31840.16152679345978824No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31310.1588380622872478No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA31190.1582292929651632No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCGGCTACTTAGCCTGGTACC30700.15574348489998427No Hit
GTCCCAGGTGATGCTGCAGAGGTCGGGCCCAGGACTGGTGAAGCCTTCAC30550.1549825232473785No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT30090.15264890751272076No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT29950.15193867663695534No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC29920.15178648430643418No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29760.150974791876988No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA29700.1506704072159457No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA29360.14894556080337257No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA28590.14503929098666288No Hit
GTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT28560.14488709865614174No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG27960.14184325204571857No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC27720.1406257134015493No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27440.1392052516500185No Hit
ATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGA27400.13900232854265698No Hit
GTGTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGG26630.13509605872594727No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGC26470.13428436629650112No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC26090.13235659677656644No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG25890.13134198123975874No Hit
TCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTGGC25700.1303780964797914No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC25010.12687767287780477No Hit
ATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACT24400.12378309549054124No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT23840.12094217198747965No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCA23820.12084071043379886No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG23090.1171373637244507No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCT23000.11668078673288723No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22950.1164271328486853No Hit
GTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGA22920.11627494051816416No Hit
GTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCA22840.11586909430344107No Hit
GCGTAGTAGTAGTAAAGGGGATAATAACCACTAGTATCACCTCTGGCACA22700.11515886342767566No Hit
ATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAA22200.11262232458565638No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21900.11110040128044481No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG21860.11089747817308326No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21850.11084674739624287No Hit
ACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGC21590.10952774719839285No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC21570.10942628564471209No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCC21410.1086145932152659No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC21400.10856386243842552No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21370.10841167010790435No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21260.10785363156266012No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21070.10688974680269278No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20660.10480978495223697No Hit
ATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTACTACT20480.10389663096911003No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATTTTGCAGTGTA20410.10354151553122733No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG20260.10278055387862155No Hit
GTACAAAATGTACCCAATCCACTCCAGGCCCCTCACTGGGTGCTGGCGGA20250.10272982310178115No Hit
TCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC19820.10054839969764458No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT19750.10019328425976187No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)