FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3574792
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT201510.5636971325884135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC193590.5415419974085205No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACTGATCACCTTCGGCCAAGGGA177950.4977912001593379No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA154820.4330881349180596No Hit
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA153390.4290879021772455No Hit
GTTTATTACTGTGCGAGAGGGTCCCATTACTATGATAGTCGTGGTTATTA130670.36553175681270406No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC124390.3479643011397586No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT122400.34239754368925523No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT111790.312717495171747No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC103160.28857623044921216No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT101180.2830374466542389No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA92530.25884023462064365No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT92430.258560498065342No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG87150.24379040794541332No Hit
GTATATGGTACGACCACTACTACTAATGTATGAAACCCACTCCAGCCCCT83210.23276878766652717No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG81870.22902031782548468No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT80030.2238731652079338No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC79840.2233416657528606No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC79180.22149540448786947No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACTGATCACCTTCGGCCAAGG73290.2050189213806006No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT73010.20423565902575588No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC72610.20311671280454918No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACC72580.2030327918379587No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA72200.2019697929278123No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT71560.20017947897388155No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG69750.19511624732292115No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC68980.19296227584709824No Hit
GTATGGTAGCTCACTGATCACCTTCGGCCAAGGGACACGACTGGAGATTA68890.19271051294732672No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC68410.19136777748187866No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT67530.18890609579522388No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA65320.18272391792305678No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA64760.18115739321336738No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT63530.17771663358315673No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG63340.17718513412808354No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG60970.17055537776743374No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT60360.1688489847800935No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA60000.16784193318100746No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA59180.16554809342753368No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG59150.1654641724609432No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC57810.16171570261990068No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA57280.1602330988768018No Hit
CATTAGTAGTAGTGGTCGTACCATATACTACGCAGACTCTGTGAAGGGCC54630.1528200801613073No Hit
TCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC54360.15206479146199275No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG53700.15021853019700168No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC53330.14918350494238544No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC52920.14803658506564857No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG52610.14716940174421336No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT50190.14039977710591273No Hit
GAGTGTTAGCAGCACCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG49360.1380779636969088No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCA49330.1379940427303183No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC49200.1376303852084261No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48910.13681914919805124No Hit
GTCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACCAGCAGAAACCTGGC48800.1365114389872194No Hit
TAGTAGTAGTGGTCGTACCATATACTACGCAGACTCTGTGAAGGGCCGAT47960.13416165192268528No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT47930.1340777309560948No Hit
GTAATAACCACGACTATCATAGTAATGGGACCCTCTCGCACAGTAATAAA47420.13265107452405622No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT46980.13142023368072883No Hit
GTGTTAGCAGCACCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT46950.13133631271413831No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG46220.12929423586043606No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC46160.12912639392725508No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA45610.12758784287309582No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG45440.12711229072908298No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA44600.12476250366454887No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG44400.12420303055394552No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA44290.12389532034311367No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA42780.11967129835805833No Hit
GCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA42420.11866424675897227No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC42300.11832856289261025No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC42260.11821666827048957No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCT41760.1168179854939812No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTCTGAAATGAACTGGG41250.11539132906194262No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCT40720.11390872531884372No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA40330.11281775275316717No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA39760.11122325438794761No Hit
ACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC39590.11074770224393474No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC39230.10974065064484871No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG38970.10901333560106434No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA38920.10887346732341349No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTC38860.1087056253902325No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG38620.10803425765750847No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA38580.10792236303538778No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGAC38560.10786641572432747No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG38390.10739086358031459No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGT37810.10576839155956486No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG37540.10501310286025034No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG37420.10467741899388831No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC37420.10467741899388831No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC37300.1043417351275263No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA36840.10305494697313856No Hit
GTGCGAGAGGGTCCCATTACTATGATAGTCGTGGTTATTACTGGGGGGGT36150.101124764741557No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC35810.1001736604535313No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)