FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154398
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCAGATCTCAGCCTGGTCACCTCCATGTAGG102490.4757245411479216No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGATGG95750.4444396996283881No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG87300.40521760603194024No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG82820.38442293392400106No Hit
GTATATTACTGTGCGAGAGATCTGGGGTACAGCGGTAAAGGATTCTGGCT78040.3622357614516909No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGATG73560.3414410893437517No Hit
ATTATGTATCGTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAAGTTCTC69760.32380275139505327No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT66290.30769616384716286No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC55730.2586801510213062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA50940.23644656187018367No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG49820.2312478938431989No Hit
GGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGATGGGATGGATCAAC48500.2251208922399668No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG48330.2243318087001566No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA44370.20595080389046033No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT43950.20400130338034103No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG41930.19462513426024347No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA40740.18910154948157212No Hit
CTACTATATGCACTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGA40620.18854454933582374No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT39490.18329946463002658No Hit
TAATTATGTATCGTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAAGTTC39310.18246396441140403No Hit
CCCTAACAGTGGTGGCACAAACTATGCACAGAACTTTCAAGGCAGGGTCA38590.1791219635369138No Hit
ATATACGGCCGTGTCGTCAGATCTCAGCCTGGTCACCTCCATGTAGGCTG38360.17805437992422943No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACC38260.17759021313610576No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTG38210.17735812974204393No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC37750.17522296251667518No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC36360.16877104416175653No Hit
CCCAGGAACAGCCCCCAAAGTTCTCATCTACGAAAATAATGAGCGACCCT36150.1677962939066969No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG35690.16566112668132815No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTCAGG35630.16538262660845396No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35180.16329387606189757No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC35110.16296895931021102No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT34560.16041604197553097No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34520.16023037526028153No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA32500.15085420614018394No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG32010.14857978887837808No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31960.14834770548431628No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA31490.14616612158013514No Hit
ATATTACTGTGCGAGAGATCTGGGGTACAGCGGTAAAGGATTCTGGCTCG29810.13836811953965794No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAG29500.13692920249647464No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA29160.13535103541685425No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA29000.13460836855585645No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG28670.13307661815504843No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28630.13289095143979895No Hit
GCCGTATATTACTGTGCGAGAGATCTGGGGTACAGCGGTAAAGGATTCTG28530.13242678465167532No Hit
GGACAGAAGGTCTCCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAA27650.12834211691618724No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG26930.12500011604169703No Hit
CCCCTGGACAACGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGT26740.12411819914426211No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC26450.12277211545870355No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGCCAGA26380.122447198707017No Hit
CATCTTCACCGACTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAC25820.1198478646935246No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT25670.11915161451133913No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA25300.11743419739528166No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGCCCGGTGATGCCCAGGGTGG25120.11659869717665909No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTTGGGTGTTCGG25050.11627378042497255No Hit
ACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAG24870.11543828020634998No Hit
GACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCCACAGGAGCCCACTCCC24860.11539186352753762No Hit
GAATAATTATGTATCGTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAAG24720.11474203002416453No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24280.11269969615642049No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC24060.11167852922254848No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT23550.1093112786031179No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC23490.1090327785302437No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA22740.1055515276193164No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC22580.10480886075831855No Hit
ACTATGCACAGAACTTTCAAGGCAGGGTCACCATGACCAGGGACACGTCC22320.10360202710919708No Hit
GCCTACATGGAGGTGACCAGGCTGAGATCTGACGACACGGCCGTATATTA22250.10327711035751054No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC22110.10262727685413743No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21970.10197744335076434No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21730.10086344305926762No Hit
ACATGGAGGTGACCAGGCTGAGATCTGACGACACGGCCGTATATTACTGT21730.10086344305926762No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC21560.10007435951945741No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)