FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154398
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCAGATCTCAGCCTGGTCACCTCCATGTAGG154200.7157451872866574No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG79050.36692384601173966No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGATGG75370.34984250820878965No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG73290.3401878390158179No Hit
GTATATTACTGTGCGAGAGATCTGGGGTACAGCGGTAAAGGATTCTGGCT63420.29437457702801434No Hit
ATTATGTATCGTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAAGTTCTC61460.2852769079807909No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGATG59380.27562223878781916No Hit
ATATACGGCCGTGTCGTCAGATCTCAGCCTGGTCACCTCCATGTAGGCTG59330.27539015539375733No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC55750.2587729843789309No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCC54560.25324939960025955No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG47390.21996864089179435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43670.20270163637359484No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA42360.19662105144917513No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT40110.18617729871639316No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG39710.18432063156389858No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA38900.1805608805800971No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC38200.17731171306323157No Hit
GGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGATGGGATGGATCAAC37850.17568712930479882No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT34190.1586986248594735No Hit
CTACTATATGCACTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTGGA33390.15498529055448435No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA32920.15280370665030324No Hit
CTGTTAGGGTTGATCCATCCCATCCACTCAAGCCGTTGTCCAGGGGCCTG32650.1515504563223694No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT32620.15141120628593233No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32590.15127195624949522No Hit
TAATTATGTATCGTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAAGTTC32560.15113270621305813No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC32050.14876545559362755No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31970.14839412216312864No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT31970.14839412216312864No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC31150.14458795450051476No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGCCAGA31120.14444870446407768No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC30930.14356678756664273No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCTC30900.14342753753020565No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG30650.14226712055989654No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30390.14106028691077507No Hit
GTGCATATAGTAGTCGGTGAAGATGTATCCAGAACCCCTGCAGGAGACCT30330.14078178683790088No Hit
CCCTAACAGTGGTGGCACAAACTATGCACAGAACTTTCAAGGCAGGGTCA29930.13892511968540633No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA29450.13669711910241283No Hit
CCCAGGAACAGCCCCCAAAGTTCTCATCTACGAAAATAATGAGCGACCCT29340.13618653563547684No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACTCAGG29120.1351653687016048No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT28960.13442270184060698No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA28820.13377286833723387No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC28640.13293736811861132No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28600.13275170140336187No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAG28280.1312663676813662No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG28180.13080220089324257No Hit
CAGTAATATACGGCCGTGTCGTCAGATCTCAGCCTGGTCACCTCCATGTA28090.1303844507839313No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCA26230.12175094852483154No Hit
CCTCCATGTAGGCTGTGGTGATGGACGTGTCCCTGGTCATGGTGACCCTG26120.12124036505789552No Hit
CCATGTAGGCTGTGGTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCT25550.11859461436559075No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG25430.1180376142198424No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG25290.1173877807164693No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT24560.11399936316316668No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTTGGGTGTTCGG24410.11330311298098122No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG24150.11209627933185976No Hit
ATATTACTGTGCGAGAGATCTGGGGTACAGCGGTAAAGGATTCTGGCTCG23380.10852219506330772No Hit
GGACAGAAGGTCTCCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAA23080.10712969469893678No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA23070.10708327802012442No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC22980.10666552791081313No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC22860.10610852776506477No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA22740.1055515276193164No Hit
TCACTGCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAG22670.10522661086762986No Hit
ACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAG22580.10480886075831855No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22290.10346277707276No Hit
TTCTCATTCACTGCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCG22240.10323069367869818No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA22190.10299861028463637No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT22090.10253444349651271No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC22010.10216311006601381No Hit
GCCGTATATTACTGTGCGAGAGATCTGGGGTACAGCGGTAAAGGATTCTG21970.10197744335076434No Hit
CCCCTGGACAACGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGT21780.10109552645332942No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)