FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1829385
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT75020.4100831700270856No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65220.35651325445436577No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCT47060.2572449211073667No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC46110.25205191908756225No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT40090.21914468523574865No Hit
CTCCTGCAGGGCCAATCAGAGTCTTGACACCTACCTAGCCTGGTACCAAC36230.19804469808159575No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34300.18749470450451927No Hit
TACCTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCAT34290.18744004132536346No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32940.1800605121393255No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT32460.17743667953984535No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA31570.1725716565949759No Hit
GTCTATTACTGCGCGGGGACTACAGAGGGAAACTTCTACTATGGTTTGGA31560.17251699341582008No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC31370.1714783930118592No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31130.1701664767121191No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30360.16595741191711968No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA30090.1644815060799121No Hit
TTATTACTGTCACCAGCGTCTCAACGGACCTTTTGGCCAGGGGACCAAAC29260.15994446220997766No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA28310.15475146019017322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT26820.14660664649595356No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26380.14420146661309677No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25950.14185094990939578No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24760.13534603158985123No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT24540.13414344164842282No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA24460.13370613621517613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24340.1330501780653061No Hit
CTCCCAGACTCCTCATCTATGGTGCAACCCACAGGGCCACTGGCATCCCA24260.13261287263205943No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA24050.13146494586978685No Hit
GTTTATTACTGTCACCAGCGTCTCAACGGACCTTTTGGCCAGGGGACCAA23200.12681857564154073No Hit
CCTCATCTATGGTGCAACCCACAGGGCCACTGGCATCCCAGCCAGGTTCA22640.12375743760881389No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT22630.12370277442965805No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22610.12359344807134638No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22490.12293748992147635No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC22390.12239085812991797No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAATCAGA22190.12129759454680125No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA22050.12053231003861953No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTCTCAAC21970.12009500460537284No Hit
TTCAAAAGGTGTCCAGTGTGAGGTGCAGGTGGTGGAGACTGGAGGAGGCT21200.11588593981037344No Hit
GTAGTAACTACATGGCGTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG20890.11419138125654249No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20670.1129887913151141No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT20380.11140355911959483No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20120.10998231646154309No Hit
ACCCACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGG19610.10719449432459542No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC19020.10396936675440106No Hit
ACCTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCATC18950.10358672450031023No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG18810.1028214399921285No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18570.10150952369238843No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)