FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842201
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT138800.48835392007813666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC119230.4194988320671198No Hit
ATCTATAGCAGGGGGAGTACCAACTACAACCCCTCCTTCAAGAGTCGAGT112920.3972977280635676No Hit
GTCTATTACTGTGCGAGAGAGATTTTCTACGGCGACAGTGATTACTGGTA99650.35060856005609736No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC93270.3281611680525058No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGA85710.3015620640482499No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT84410.2969881440475181No Hit
GTCTCTGGTGGCTCCATCAGTCGTTACTACTGGAACTGGATCCGGCAGTC80250.2823516000451763No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT69970.24618244803938918No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTG67880.23882899203821265No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT64060.2253887040360622No Hit
TCATTAGACACGTCCAATCAGTTCTCCCTGAAGCTGACCTCTGTGACCGC63520.2234887680357582No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT59910.21078734403372598No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC57260.20146358403223416No Hit
ACATAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAACA54320.1911194880305791No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54000.18999360003039897No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA53500.18823440003011752No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53200.18717888002994862No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52960.18633446402981352No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA52290.18397713602943633No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC51810.18228830402916613No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT51250.18031800002885087No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCACTTACGTGGCCTGGTTCCAAC50780.1786643520285863No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA47000.16536480002645837No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45970.16174084802587854No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA45910.16152974402584477No Hit
TCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC45730.16089643202574344No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAACGTAGCAAT45010.1583631840253381No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG44630.1570261920251242No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44470.15646324802503414No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA44110.15519662402483148No Hit
GTTTATTACTGTCAACAACGTAGCAATTGGCCTCCGCTCACTTTCGGCGG43950.15463368002474137No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41940.14756169602360986No Hit
TTATTACTGTCAACAACGTAGCAATTGGCCTCCGCTCACTTTCGGCGGAG41760.14692838402350852No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA41330.1454154720232665No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA41210.14499326402319893No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT40710.14323406402291744No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40500.14249520002279922No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT40330.14189707202270352No Hit
TACGTGGCCTGGTTCCAACATAAACCTGGCCAGGCTCCCAGGCTCCTCAT40120.1411582080225853No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC39250.13809720002209555No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT38350.13493064002158892No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTCGTTACTACTGGAACTGGA38190.13436769602149884No Hit
GTCAACAACGTAGCAATTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAG38130.13415659202146507No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA37550.13211592002113853No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC37380.13151779202104286No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG36390.1280345760204855No Hit
GTCAGAGTGTTCGCACTTACGTGGCCTGGTTCCAACATAAACCTGGCCAG35960.12652166402024348No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT35810.12599390402015903No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC35690.12557169602009147No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC34990.12310881601969742No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG34860.12265142401962421No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA33530.11797195201887552No Hit
CCTCATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCA33140.11659977601865598No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT32840.11554425601848707No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTCGTTACTACTGGA32580.11462947201834073No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC32200.1132924800181268No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC32030.1126943520180311No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG31890.11220177601795227No Hit
GTCCTGGACCTCCTGTGCACGAACATGAAACATCTGTGGTTCTTCCTTCT31570.11107588801777214No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCC30730.10812043201729926No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG30530.10741675201718667No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA30080.10583347201693337No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30060.10576310401692209No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA29590.1041094560166575No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC29270.10298356801647737No Hit
CTATTACTGTGCGAGAGAGATTTTCTACGGCGACAGTGATTACTGGTACT28730.10108363201617339No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTATGTTGGAACCAGGC28680.10090771201614523No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)