FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185533
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72660.612888886264659No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT70210.592223076034155No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT58710.4952202933195449RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT47770.40294112437190704TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT46620.39324084610044596No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT37550.31673517312466204RNA PCR Primer, Index 39 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36840.31074630567010786No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCT33220.2802115166764654RNA PCR Primer, Index 11 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32460.27380089799271723No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG30460.25693084882495887RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24410.20589895009249004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG21270.1794129728991095No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT20810.1755328615905251RNA PCR Primer, Index 39 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGCTTG19950.16827874044838903TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCG17780.1499747371013713TruSeq Adapter, Index 6 (96% over 33bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA16560.13968400710903872No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA15080.1272001707248976No Hit
TATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA14380.12129565351618218TruSeq Adapter, Index 16 (97% over 34bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGCT14110.1190181968785348TruSeq Adapter, Index 11 (96% over 26bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA13940.11758424269927535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13490.11378848163652973No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA13150.11092057327801082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA13080.11033012155713928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12360.1042569038567463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)