FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005112952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671127
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76700.4589716999366296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.24378757568993858No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT37390.22374122373703492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32780.1961550498555765No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT30530.18269108212601437RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT25200.15079643857109604No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT23320.13954654553483967TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22750.13613567371001725No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT20530.1228512255501826RNA PCR Primer, Index 39 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18190.10884869911143796No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCT17540.10495910843400891RNA PCR Primer, Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)