FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932412
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC107560.36679702579310136No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT97530.33259310083303434No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTC97170.3313654425094427No Hit
GTGCATATACATCAAGCGCCAACACCCATGTGTTATTCGGCGGAGGGACC91220.311074978550081No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCGAGAGCCTGGGGAGCGGGGG90170.30749430843960535No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA89470.3051071950326216No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAACACATGGGTGTTG89360.3047320772115242No Hit
GCATATACATCAAGCGCCAACACCCATGTGTTATTCGGCGGAGGGACCAG86910.29637718028708104No Hit
GTATATGCACTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGA79150.26991432308966135No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAACAC76120.25958153219943175No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA75580.25774004471404427No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT73680.25126073689508843No Hit
GATTATTACTGCAGTGCATATACATCAAGCGCCAACACCCATGTGTTATT70820.24150767354655486No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCT70470.24031411684306297No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT69680.23762008885518135No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC68600.2339371138844064No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA68240.23270945556081477No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67440.22998132595283335No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT67110.22885597248954107No Hit
GTACATGGAATTGAGCGGCCTGAGATTTGAGGACACGGCCGTGTATTATT66670.22735550120515124No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT64970.22155822578819073No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA64930.2214218193077917No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA64310.21930751886160607No Hit
TTCCAGGACAGAGTCACTATGACCAGCGACACGTCCACCAACACACTGTA63860.21777294595711652No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63290.21582915361142976No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT63160.2153858325501328No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGCCACAGGGTCCTGGGCCCAGTC62430.21289641428284975No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT61440.20952035389297274No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA60530.20641710646389388No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG59530.20300694445391712No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC58800.20051752618663407No Hit
TCCTCACCCTCCTCACTCAGGCCACAGGGTCCTGGGCCCAGTCTGCCCTG57860.1973119738972559No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG55100.18789992674972003No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCAGTGCATAT54250.18500128904123977No Hit
TCCTCACTCAGGCCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT53800.18346671613675022No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA52180.17794225368058786No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT50590.1725200960847248No Hit
GTATTATTGTGCTTTTAGTAGTGGCTGGTGGGCGTTAGGCGTCTGGGGCC50340.1716675555822306No Hit
TTACTGCAGTGCATATACATCAAGCGCCAACACCCATGTGTTATTCGGCG50210.17122423452093363No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG50070.17074681183953688No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAACACATGGGTGTTGG49920.17023528753804038No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA49630.1692463405551471No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG48800.16641590608686638No Hit
ATGTATATGCACTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCA48510.16542695910397312No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT45090.15376420502985255No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGCAAGTTTCCTGTA44830.1528775629072586No Hit
CCCCTGGACAAGGGCCTGAGTGGATGGGCATGATCAACCCTCCTGGTGGG44650.15226373374546276No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC44390.15137709162286883No Hit
TCCATGTACAGTGTGTTGGTGGACGTGTCGCTGGTCATAGTGACTCTGTC44290.15103607542187114No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC43800.14936509603698253No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC43490.14830794581388973No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC43290.14762591341189438No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG43180.14725079559079693No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA43000.14663696642900112No Hit
GGGTACGTGCCAAGCATCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCG42670.14551161296570878No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG42390.14455676760291528No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT41700.1422037558160313No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGGCCCCAA41660.14206734933563223No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT41630.14196504447533292No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGCCACAGGGTCCT41490.1414876217939362No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA41380.14111250397283875No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA40520.1381797646442587No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG40120.136815699840268No Hit
ATAATACACGGCCGTGTCCTCAAATCTCAGGCCGCTCAATTCCATGTACA40030.1365087852593701No Hit
GGGTGCGGCAGGCCCCTGGACAAGGGCCTGAGTGGATGGGCATGATCAAC39380.1342921799528852No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC38110.12996127420021472No Hit
GTGTATTATTGTGCTTTTAGTAGTGGCTGGTGGGCGTTAGGCGTCTGGGG37880.12917693693792004No Hit
CTCATGATGTATGATGTCACTAGTCGGCCCTCAGGGGTTTCTGATCGCTT37620.12829029481532608No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG37290.12716494135203374No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAAC36100.1231068485601614No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA36080.12303864531996186No Hit
TGTATATGCACTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAG36010.12279993397926348No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATGGTCAGGG36000.12276583235916372No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT35440.12085614163357672No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35350.12054922705267881No Hit
CCAACACACTGTACATGGAATTGAGCGGCCTGAGATTTGAGGACACGGCC34950.11918516224868811No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT34600.11799160554519625No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC34530.11775289420449786No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA34490.11761648772409879No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA34350.11713906504270204No Hit
GTATGATGTCACTAGTCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCT34300.1169685569422032No Hit
TTATTACTGCAGTGCATATACATCAAGCGCCAACACCCATGTGTTATTCG33730.11502476459651645No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT33410.11393351275332388No Hit
GAATAACACATGGGTGTTGGCGCTTGATGTATATGCACTGCAGTAATAAT32970.1124330414689341No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG32830.11195561878753735No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC32760.11171690744683899No Hit
GGCCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGT32650.11134178962574152No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGCC32650.11134178962574152No Hit
GTGTTATTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGC32540.11096667180464409No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG32490.11079616370414526No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG32210.10984131834135176No Hit
CTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATGGTCAG32060.10932979403985524No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCGGG31960.10898877783885758No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA31890.10875006649815919No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG31610.10779522113536572No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG31600.10776111951526593No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA31590.10772701789516616No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGCCACAGG31410.10711318873337033No Hit
CAATAATACACGGCCGTGTCCTCAAATCTCAGGCCGCTCAATTCCATGTA31380.10701088387307105No Hit
CATTCACCACCGACCACATGCACTGGGTGCGGCAGGCCCCTGGACAAGGG31360.1069426806328715No Hit
TATATGCACTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAG31350.10690857901277173No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTT30940.10551041258868125No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT30860.10523759962788312No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA30570.10424865264498986No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT30460.1038735348238924No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT30340.10346431538269521No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT29840.10175923437770681No Hit
CTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACC29740.10141821817670914No Hit
GTTCTTCACCATGGACTGGACCTGGAGGGTCTTCTGCTTGCTGGCTGTAG29440.10039516957371611No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC29430.10036106795361635No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG29410.10029286471341681No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)