FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005112956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932412
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTC114700.39114558254433557No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC107220.36563757070970926No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT106000.3614771730575376No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCGAGAGCCTGGGGAGCGGGGG100050.34118670909817583No Hit
GTGCATATACATCAAGCGCCAACACCCATGTGTTATTCGGCGGAGGGACC87560.298593785593566No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT86380.29456979442179343No Hit
GCATATACATCAAGCGCCAACACCCATGTGTTATTCGGCGGAGGGACCAG82040.2797696912984942No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA81350.27741667951161025No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCT77870.26554931571689105No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAACACATGGGTGTTG76780.2618322391260164No Hit
GATTATTACTGCAGTGCATATACATCAAGCGCCAACACCCATGTGTTATT70750.24126896220585647No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA70130.23915466175967087No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGCCACAGGGTCCTGGGCCCAGTC69850.23819981639687737No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT68780.23455094304620222No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT67170.22906058221013964No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC66160.22561631858006312No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAACAC66100.2254117088594645No Hit
GTATATGCACTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGA65540.22350201813387752No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT62340.21258949970195182No Hit
GTACATGGAATTGAGCGGCCTGAGATTTGAGGACACGGCCGTGTATTATT61540.20986137009397043No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG61480.2096567603733718No Hit
TCCTCACTCAGGCCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT60690.20696273238549018No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA58950.2010290504881306No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC58550.19966498568413987No Hit
TCCTCACCCTCCTCACTCAGGCCACAGGGTCCTGGGCCCAGTCTGCCCTG58500.19949447758364106No Hit
TTCCAGGACAGAGTCACTATGACCAGCGACACGTCCACCAACACACTGTA57910.19748248199775476No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT57790.19707326255655752No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA57550.19625482367416314No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA57070.19461794590937426No Hit
TCCATGTACAGTGTGTTGGTGGACGTGTCGCTGGTCATAGTGACTCTGTC56780.193628998926481No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCAGTGCATAT56700.19335618596568288No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA56550.19284466166418635No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA55990.19093497093859935No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG53750.18329620803625138No Hit
ATAATACACGGCCGTGTCCTCAAATCTCAGGCCGCTCAATTCCATGTACA53160.1812842124503651No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA52890.18036346870767136No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA52400.17869248932278275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52290.1783173715016853No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG50900.1735772463078176No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGC50030.1706104053591378No Hit
TTACTGCAGTGCATATACATCAAGCGCCAACACCCATGTGTTATTCGGCG48770.16631360122656708No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG48500.16539285748387336No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC48380.16498363804267613No Hit
GTATTATTGTGCTTTTAGTAGTGGCTGGTGGGCGTTAGGCGTCTGGGGCC48360.1649154348024766No Hit
GGGTACGTGCCAAGCATCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCG47290.16126656145180146No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT47210.1609937484910033No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGGCCCCAA47000.16027761446890817No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG46270.15778819620162515No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAACACATGGGTGTTGG46160.1574130783805277No Hit
CTCATGATGTATGATGTCACTAGTCGGCCCTCAGGGGTTTCTGATCGCTT45810.15621952167703584No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGCCACAGGGTCCT44600.15209322564496394No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT44130.15049044950027488No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGCAAGTTTCCTGTA44050.15021763653947673No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG43900.14970611223798022No Hit
GGCCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGT43630.14878536849528648No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT43300.14766001503199414No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC43200.14731899883099647No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA43060.14684157614959972No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT42700.14561391782600808No Hit
ATGTATATGCACTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCA41910.14291988983812642No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41230.14060097967134222No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT40670.13869128894575525No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA40370.1376682403427622No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT39860.13592905771767405No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC39860.13592905771767405No Hit
CCCCTGGACAAGGGCCTGAGTGGATGGGCATGATCAACCCTCCTGGTGGG39790.1356903463769757No Hit
CAATAATACACGGCCGTGTCCTCAAATCTCAGGCCGCTCAATTCCATGTA39530.13480370425438173No Hit
GTATGATGTCACTAGTCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCT38950.1328258102885952No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT38490.13125713576400588No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT38210.13030229040121238No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA38000.12958615637911725No Hit
GGGTGCGGCAGGCCCCTGGACAAGGGCCTGAGTGGATGGGCATGATCAAC37230.12696033163143514No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG36740.12528935224654653No Hit
GTGTATTATTGTGCTTTTAGTAGTGGCTGGTGGGCGTTAGGCGTCTGGGG36730.12525525062644674No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT36650.12498243766564862No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTT35450.12089024325367649No Hit
GTCCTGGAACTTCTGTGCGTAGCTCGTGCTCCCACCAGGAGGGTTGATCA35230.12014000761148161No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGCC35150.11986719465068346No Hit
TTATTACTGCAGTGCATATACATCAAGCGCCAACACCCATGTGTTATTCG34660.11819621526579485No Hit
GTGTTATTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGC34170.11652523588090624No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG33740.1150588662166162No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGCCACAGG33530.1143427321945211No Hit
AGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG33460.11410402085382274No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG33410.11393351275332388No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33190.113183277111129No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC32930.11229663498853502No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA32870.11209202526793642No Hit
GCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC31740.10823854219666268No Hit
CTGTTGAACTGCTCCTCCCGTGGCTTTGTCTTGGCATTATGCACCTCCAT31400.10707908711327058No Hit
CCAACACACTGTACATGGAATTGAGCGGCCTGAGATTTGAGGACACGGCC31190.10636295309117547No Hit
ATACACGGCCGTGTCCTCAAATCTCAGGCCGCTCAATTCCATGTACAGTG31170.10629474985097591No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT30960.1055786158288808No Hit
CTTCTGTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGA30830.10513529476758382No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC30490.10397583968419172No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG30140.10278228298069986No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG30010.10233896191940287No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29950.10213435219880428No Hit
TGTATGATGTCACTAGTCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGC29600.10094079549531239No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT29480.10053157605411518No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)