FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005112971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781144
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT110300.39659938500128006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC90500.3254056604044954No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCCCTTTTCGGCCCTGGGACCA89440.321594279188708No Hit
GTGTACTACTGTGCAAGAGACAAGTCCATAGCAGCAGGTGGTAACTGGTT78930.28380407486990966No Hit
GTAGTACACAGCCGTGTCCTCGGCGCTTAGACTGTTCATTTGCAGATACA71030.2553984978843239No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64660.23249425416303507No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC62610.22512318671740836No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT61150.2198735484390596No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT58360.20984170542769448No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT55900.20099642449294247No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT51760.18611046389543295No Hit
GTATGGTAGCTCACCCCTTTTCGGCCCTGGGACCAAAGTGGATATGAAAC50310.18089678204364823No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49220.17697753154816867No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT46250.16629847285865096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42890.154217113533136No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41380.14878769312196707No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA40470.1455156583046401No Hit
CATCTATGCTGCATTCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG39850.14328635985767008No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT39540.14217171063418507No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT36840.13246347546189624No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36220.13023417701492623No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36010.12947909205708155No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCCCTTTTCGGCCCTGGGAC34870.12538005942878183No Hit
CTCCTGCAGGACCAGTCAGACTGTGAGTAGCAGCTACTTAGCCTGGTACC34350.12351032524745213No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA33830.12164059106612242No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33450.12027424685668918No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC32760.11779325342377095No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG32160.11563586782992896No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA32020.11513247785803252No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGACCAGTCAGA31910.11473695716582816No Hit
GTCTAAGCGCCGAGGACACGGCTGTGTACTACTGTGCAAGAGACAAGTCC30450.1094873188874794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30440.10945136246091536No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCT29870.1074018461467655No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA29840.1072939768670734No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC29590.1063950662029726No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC28880.10384215991692627No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA28530.10258368498718513No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC28150.1012173407777519No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)