FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005112995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005112995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538579
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA135980.5356540017072543No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG91480.36035908277819995No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC85260.3358571862447456No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA82750.32596976497481467No Hit
GTGTATTACTGTGCGAGAGAGCGTATTACTATGGTTCGGGGAGTCCCTAA68610.27026931208364996No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG67910.267511863920721No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA66450.2617606148951835No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTA64480.25400036792236913No Hit
TAGTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC63140.2487218242961909No Hit
GTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA60230.23725871836172913No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT59120.2328861934176561No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA56520.2226442430982057No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC54810.21590819115733642No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54600.21508095670845778No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53970.2125992533618217No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC53700.2115356662132634No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50950.20070283414461398No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATG50700.19971803122928222No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT49960.19680301459990018No Hit
TAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCT49050.19321833198809255No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA49040.1931789398714793No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC48440.19081541287468304No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA48420.1907366286414565No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA47710.18793978836191427No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT47130.1856550455983446No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC46490.18313395013509526No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC44860.17671303512713216No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG44040.17348288156484395No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG43120.16985880683642304No Hit
GGATAGTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCG41400.1630833627789405No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39720.15646548718791103No Hit
GTATATGGTACTACTACTACTACTAATGTGTGAAACCCACTCCAGCCCCT39470.15548068427257927No Hit
CATTAGTAGTAGTAGTAGTACCATATACTACGCAGACTCTGTGAAGGGCC39370.15508676310644653No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC39220.15449588135724748No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT38850.1530383730425565No Hit
AAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC38340.1510293750952797No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36560.14401757833811749No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG36550.14397818622150424No Hit
CCCCTGTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCT36460.1436236571719848No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAACTGTGCACTGGTAC36210.14263885425665304No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTAC35370.13932991646113832No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT35000.13787240814644727No Hit
GTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAG34510.13594219443239702No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC34500.13590280231578375No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC34030.13405137283496002No Hit
GTGCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG33820.13322413838608135No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG33660.132593864520269No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCA33420.13164845372155054No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC33110.13042729810653914No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT32610.1284576922758756No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA32500.12802437899312963No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG32500.12802437899312963No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGG31920.12573963622955991No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG31320.12337610923276368No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31150.1227064432503381No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT31090.12247009055065847No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30800.12132771916887361No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA30750.12113075858580725No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA30610.12057926895322148No Hit
TCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGC30430.11987021085418259No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATTATGAT30060.11841270253949158No Hit
CTCCAGAGACAATGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA29940.11793999714013234No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA29510.11624613612576169No Hit
CTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT29500.11620674400914842No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29280.11534011744365646No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG29280.11534011744365646No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTT29120.11470984357784414No Hit
GTGTGGGATAGTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCT28430.11199178753152847No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGGTATTCGGCG28420.1119523954149152No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC27380.10785561528713504No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG26990.1063193227392175No Hit
CCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTACCTGTGGGGG26910.10600418580631132No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC26900.10596479368969805No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA26860.10580722522324497No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG26830.10568904887340518No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTTACTATAGCATGAACTGGG26640.10494059865775301No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26230.10332552187660893No Hit
GTTAGGGACTCCCCGAACCATAGTAATACGCTCTCTCGCACAGTAATACA25940.10218315049482407No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCA25760.1014740923957852No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25730.10135591604594539No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG25670.10111956334626576No Hit
CTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG25640.10100138699642597No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG25580.10076503429674634No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG25440.10021354466416053No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)