FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005113002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839809
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGCTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGG144660.5094004561574388No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC78260.27558191413577465No Hit
CTACAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG75960.26748277789104835No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA71530.2518831372109885No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA68290.24047391919667835No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC67790.23871323740434655No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT62480.22001479676978275No Hit
GGGTTAACAATGATGGCAGCCACTACAAGGGGGACGGGATCCCTGATCGC62380.2196626604113164No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT59660.21008455146103136No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG59200.2084647242120861No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA54400.19156217900570074No Hit
ACTCTGGACAGTGGTCACAGCACCTACGCCATCGCATGGCTTCAGCAGCA54090.19047055629445503No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCGAAAGCCAGTG52790.18589278363439232No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT52190.18377996548359415No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC51820.1824770609572686No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC51520.1814206518818695No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG51090.1799064655404642No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49640.17480048834270193No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA48890.1721594656542042No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48780.17177211565989123No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG46460.16360255214347164No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG45890.16159537490021336No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA45620.16064460673235417No Hit
GTATCAACGCAGAGTACGGGACTCAAAAACCACACCCCTCCTTGGGAGAA44970.15835572040232285No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG44030.15504563863273904No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCG42110.1482846205501849No Hit
TTGTTAACCCTCATCAAATAGCGAGGGCCCTTCTCTGGCTGCTGCTGAAG41740.14698171602385934No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA41050.14455197515044146No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCATCGTCTCCTCAGCTTCC39640.13958685249606576No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC39120.1377557434320407No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38730.13638241163402187No Hit
GGGTGGATCAGCGCTTACAATGGCGACACAAGTTATTCAGAAAAATTCCA38730.13638241163402187No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA38710.13631198436232858No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC38670.13617112981894205No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGCGCTTACAATGGC38180.1344456616624569No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA37240.13113557989287308No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT36780.1295157526439278No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA35520.12507883452725166No Hit
TTCCAGGACAGAGTCATCCTGACCACAGAGAGATCCACTAAAACAGCCTA35160.12381114363677276No Hit
CACCTGCACTCTGGACAGTGGTCACAGCACCTACGCCATCGCATGGCTTC34850.12271952092552704No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTG34690.12215610275198086No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT34670.12208567548028758No Hit
CTATTTGATGAGGGTTAACAATGATGGCAGCCACTACAAGGGGGACGGGA34420.12120533458412168No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT34100.12007849823702933No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT33850.1191981573408634No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCC33450.11778961190699797No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33430.1177191846353047No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT33310.11729662100514507No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC33240.11705012555421862No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA33020.11627542556559262No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAACTTATCTTG32630.11490209376757379No Hit
GAAGTACACGGCCGTGTCGTCTGATCTCAGGCGCCTCAGCTCCATGTAGG32620.11486688013172718No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG32610.11483166649588054No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32180.11331748015447518No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC32120.11310619833939535No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC31820.11204978926399628No Hit
GTCTGGCGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA31250.110042612020738No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT31210.10990175747735147No Hit
GGTTACAGGTTCAGCACGTACGGTATAAGCTGGGTGCGGCAGGCCCCTGG31170.10976090293396493No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG31060.10937355293965192No Hit
CTCCCAGGTTCAGTTGGTGCAGTCTGGCGCTGAGGTGAAGAAGCCTGGGG30970.1090566302170322No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG30790.10842278477179275No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT30040.10578176208329504No Hit
GTGTACTTCTGTGCGAGAGATGAGACGTTCGATGTGCTCGACGGTATGGA30000.10564090753990849No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA29330.10328159393818387No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG29280.1031055257589507No Hit
ACCTACGCCATCGCATGGCTTCAGCAGCAGCCAGAGAAGGGCCCTCGCTA29260.1030350984872574No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCC29040.10226039849863143No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGTCGAAAGCCAGTGT28900.1017674075967785No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC28690.10102792124399916No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28640.10085185306476599No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA28540.10049971670629962No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)