FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005113026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927888
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT137950.47115873284770454No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC119200.40711939800975994No Hit
ATTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCAA92630.3163713912554032No Hit
ACAGTACTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAAGCAATGGA88770.30318782685676504No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT77400.2643543742110354No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC63120.2155820167984568No Hit
ATAATAAACGGCCGTGTCGTTAGATGTCAAACTGCTCAGGTTCATGTAGG62490.21343029514790182No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT61700.21073210450672977No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG61490.21001486395654478No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC58310.1991537927680294No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC58160.1986414780893258No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA57950.19792423753914085No Hit
ACTATATCCACTGGGTGCGACAGGCCCCTGGACAGGGACTTGAGTGGATG56440.1927669364401917No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA55740.19037613460624178No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA54530.18624346286469973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT54280.18538960506686047No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG54220.18518467919537906No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53940.18422835846179908No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT53510.18275972304951554No Hit
GATCTATGTTGCATCCACTTTGCAAAGTGGGGTCCAATCAAAGTTCAGTG53410.18241817993037984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53040.18115447038957774No Hit
GTCTCCATCCTCCCTGTCTGCATCTATTGGAGACAGAGTCACCATCACTT50050.1709423311274202No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCGCAGTGAAGGTCTCCTGCA49950.1706007880082845No Hit
GAGTACTGTAGCTCTGTTGGCAGTAGTAAGTTGCAAAGTCTTCAAGTTGG49780.17002016470575376No Hit
CTACAGTACTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAAGCAATG49510.1690979982840874No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49030.167458591312236No Hit
CTATATCCACTGGGTGCGACAGGCCCCTGGACAGGGACTTGAGTGGATGG46170.15769045810495483No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA45750.15625597700458488No Hit
GAGCTACAGTACTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAAGCA45710.1561193597569306No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45330.1548214959042149No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT44600.15232823113452426No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA44110.15065466985075932No Hit
GTACTGTAGCTCTGTTGGCAGTAGTAAGTTGCAAAGTCTTCAAGTTGGAG43730.14935680599804366No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG43460.14843463957637723No Hit
CATGTAGGCTGTGCTGATGGACGTGTCCCAGGTCATGGTCACCCTGCCCT43090.14717093003557513No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42950.14669276966878517No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC42650.14566814031137804No Hit
ATGTTGCATCCACTTTGCAAAGTGGGGTCCAATCAAAGTTCAGTGGCAGT42010.1434822643489095No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT41820.1428333324225517No Hit
GCCTACATGAACCTGAGCAGTTTGACATCTAACGACACGGCCGTTTATTA41150.14054499352434247No Hit
GTTCCATTGCTTTCCACCTTGGTCCCTTGGCCGAATGTCCACGGAGTACT40600.1386665063690961No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA39660.13545600104922045No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC38920.13292858196761623No Hit
GTCTGCATCTATTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA38320.13087932325280202No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG36320.12404846087008792No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT36290.12394599793434723No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC36280.12391184362243364No Hit
CCCCTGGACAGGGACTTGAGTGGATGGCTTGGATCAACCCTAAAAGTGGT36260.12384353499860651No Hit
GTTTATTATTGTGCGAGAGCTGGGGGCACCAGCTCGTCATTTTTCCCCAC36060.1231604487603351No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC35920.12268228839354511No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC35350.1207354926144716No Hit
CTGTAGCTCTGTTGGCAGTAGTAAGTTGCAAAGTCTTCAAGTTGGAGATT35230.12032564087150874No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG35170.12012071500002731No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG34520.11790068472564526No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC34040.11626127775379387No Hit
CCCTAAAAGTGGTGACACAAAGTATGCACAGAAGTTTCAGGGCAGGGTGA33730.11520249408447317No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG33390.11404124747941179No Hit
GATTTCACTCTCACCATCAGCAATCTCCAACTTGAAGACTTTGCAACTTA33260.11359724142453535No Hit
CTCCTGATCTATGTTGCATCCACTTTGCAAAGTGGGGTCCAATCAAAGTT32980.11264092069095538No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC32520.11106982234293115No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32500.111001513719104No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCATTGCTTTCCACCTTGGTCC31860.1088156377566355No Hit
CTTGAAGACTTTGCAACTTACTACTGCCAACAGAGCTACAGTACTCCGTG30930.10563928674867346No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT30680.10478542895083418No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG30530.10427311427213062No Hit
ACATGAACCTGAGCAGTTTGACATCTAACGACACGGCCGTTTATTATTGT30060.10266786161219282No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA29560.10096014601651429No Hit
CTATTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGC29420.1004819856497243No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC29380.10034536840207002No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)