FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005113113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162516
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC171470.5421948853381295No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT167690.5302423766393594No Hit
GTCTATGGTGGATCTTTCAGTGGTTATTATTGGAGTTGGATCCGTCAGTC108690.3436820556797183No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAGCATTAGCAC108180.3420694156171858No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG92390.2921408144654446No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG90270.2854372910682507No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC85160.26927927004954283No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG83650.26450459064871135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT80350.25406986083232463No Hit
CTCCTGATCTATGCTGCAACCGCTTTGCAAAGTGGGGTCCCACCTAGGTT76740.24265489882106522No Hit
ATTTAAATTGGTATCAGCAAAAACCAGGGAGAGCCCCTAAACTCCTGATC74100.23430711496795592No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG71770.22693956330971923No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT70810.2239040055449522No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC69500.21976173401178048No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT68980.21811747355586503No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT68120.21539811972492787No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG66590.2105601995373304No Hit
GAGTTACAGTACTCCTCGGATGTTCACTCCCGGCCCTGGGACTAAAGTGG64600.20426774125411543No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAGCTTCAGGGAGA63400.2004732940481566No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG55850.17659989704399914No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA55280.1747975346211687No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC55200.1745445714741048No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA54240.17150901370933774No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG53740.16992799404018827No Hit
GTACTGTAACTCTGTTGACAGTGGTAAGTTGCAAAATCTTCAGGTTGCAG53180.16815725201074083No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT52780.16689243627542122No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG52660.16651299155482532No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG50190.1587027543892268No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGA48860.15449724206928914No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAGCTTCAGGGA47600.1505130725030324No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC47310.1495960810949257No Hit
TTACAGTACTCCTCGGATGTTCACTCCCGGCCCTGGGACTAAAGTGGATA46530.14712969041105248No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG46450.14687672726398854No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT46200.1460862174294138No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG44260.13995186111311375No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC43400.1372325072821766No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC42670.13492421856521833No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC42490.1343550514843245No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT40980.12958037208349302No Hit
ATCTAGAGCAGTGGGGCGAAGGACTGTTGAAGCCTTCGGAGACCCTGTCC40810.1290428253959822No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTAGTCC40730.12878986224891825No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG40340.12755666690698167No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA40140.12692425903932186No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC39900.1261653695981301No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC39830.12594402684444916No Hit
CTTTAGTCCCAGGGCCGGGAGTGAACATCCGAGGAGTACTGTAACTCTGT39680.12546972094370432No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA39450.12474245189589554No Hit
AGTCAGAGCATTAGCACCTATTTAAATTGGTATCAGCAAAAACCAGGGAG39420.12464759071574658No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTAGTCCCAGGGCCGGGAGTG39150.12379384009440583No Hit
CCCCTAAACTCCTGATCTATGCTGCAACCGCTTTGCAAAGTGGGGTCCCA38980.12325629340689502No Hit
CTGTAACTCTGTTGACAGTGGTAAGTTGCAAAATCTTCAGGTTGCAGACT38970.12322467301351203No Hit
GATCTATGCTGCAACCGCTTTGCAAAGTGGGGTCCCACCTAGGTTCAGTG38640.12218120003187335No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC38580.12199147767157541No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC38020.12022073564212797No Hit
ACTTACCACTGTCAACAGAGTTACAGTACTCCTCGGATGTTCACTCCCGG37910.11987291131491508No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT37880.11977805013476611No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA36900.11667925158323308No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36880.1166160107964671No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT36620.11579388056850938No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG36500.1154144358479135No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA36080.11408637932582791No Hit
GTTACAGTACTCCTCGGATGTTCACTCCCGGCCCTGGGACTAAAGTGGAT35940.11364369381846606No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCT35410.11196781296916758No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG35060.11086109920076294No Hit
ATACACAGCCGTGTCCGCGGCGGTCACAGACCTCAGCTTCAGGGAGAACT34620.10946980189191137No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA34400.1087741532374856No Hit
CTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGAGCATTAGC33860.10706665199480413No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA33740.10668720727420825No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC33500.1059283178330165No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAAC33340.10542239153888866No Hit
GCATTAGCACCTATTTAAATTGGTATCAGCAAAAACCAGGGAGAGCCCCT33250.10513780799844175No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT33110.10469512249107989No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG33070.10456864091754792No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT32900.1040310942300371No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTC32730.10349354754252627No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGAC32580.10301924164178142No Hit
CATTAGCACCTATTTAAATTGGTATCAGCAAAAACCAGGGAGAGCCCCTA32580.10301924164178142No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG31840.10067933253144015No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)