FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005113116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138124
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT158370.5046645702974134No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC131740.41980495353274755No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCT111080.3539694416154365No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC101080.3221032693418106No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT82770.26375630790880156No Hit
CTCCTGCAGGGCCAATCAGAGTCTTGACACCTACCTAGCCTGGTACCAAC78000.24855614373428203No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC76780.24466847071689968No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT74230.23654259678712505No Hit
GTCTATTACTGCGCGGGGACTACAGAGGGAAACTTCTACTATGGTTTGGA74170.23635139975348327No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA71850.2289584477860021No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT70000.2230632059153813No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC68500.2182832800743374No Hit
TACCTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCAT66840.2129934954769155No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA66310.21130458834641336No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC65630.2091376886318068No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC64960.20700265508947382No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA63610.20270072183253435No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT62280.19846252092014208No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA59290.18893453541032795No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG59280.18890266923805432No Hit
TTATTACTGTCACCAGCGTCTCAACGGACCTTTTGGCCAGGGGACCAAAC56960.1815097172705731No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT55920.17819563535411603No Hit
CTCCCAGACTCCTCATCTATGGTGCAACCCACAGGGCCACTGGCATCCCA55450.1766979252572556No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT54360.17322451247943038No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT53510.1705158878361722No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT53210.16955990266796342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52200.16634141926832718No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG50960.16239001390639757No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC49650.1582155453385526No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAATCAGA49050.15630357500213504No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA48920.1558893147625779No Hit
CCTCATCTATGGTGCAACCCACAGGGCCACTGGCATCCCAGCCAGGTTCA48490.154519069354812No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48010.15298949308567794No Hit
GTTTATTACTGTCACCAGCGTCTCAACGGACCTTTTGGCCAGGGGACCAA47470.15126871978290213No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA46940.14957981265239997No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTCTCAAC46090.14687118800914176No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA45710.145660273462744No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT43860.1397650315921232No Hit
GTAGTAACTACATGGCGTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG43540.13874531407936716No Hit
TTCAAAAGGTGTCCAGTGTGAGGTGCAGGTGGTGGAGACTGGAGGAGGCT43190.13762999804979026No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC42910.13673774522612875No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTG42670.1359729570915617No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC41970.13374232503240788No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG41580.1324995443137365No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG41410.13195781938508483No Hit
CATCTATGGTGCAACCCACAGGGCCACTGGCATCCCAGCCAGGTTCAGTG41020.13071503866641343No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT40420.12880306832999588No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC40310.128452540434986No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG39720.12657243627084208No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA39680.12644497158174756No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGGT38070.12131451784569379No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC37420.11924321664790812No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC37380.11911575195881362No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC36780.11720378162239604No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA36580.11656645817692353No Hit
ACCTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGACTCCTCATC35850.11424022760094885No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA35390.11277438367636204No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG35150.11200959554179503No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC34670.11048001927266098No Hit
ACCCACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGG34540.11006575903310385No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC33790.10767579611258192No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG33380.10636928304936324No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG33190.10576382577616435No Hit
GTGCAACCCACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGG32760.10439358036839844No Hit
CTCCTCATCTATGGTGCAACCCACAGGGCCACTGGCATCCCAGCCAGGTT32750.10436171419612482No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT32010.1020036174478765No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG31970.101876152758782No Hit
GAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTGT31710.10104763227966772No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)