FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005113147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238836
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT195840.6046616747498176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC189370.584685362272125No Hit
GTATTACTGTCAGCAGTACGGTACGTCACGCATCACTTTCGGCGGAGGGA168720.5209278889082374No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC122140.3771107891847565No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGACTGGAGCCTGAAGATT107000.3303656004811605No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATAGTGAGAGT94180.29078347900295043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC91500.2825089013460391No Hit
CTCCTGCACGGCCAGCGAGACTATTGCCAGTGGCTACGTAGCCTGGCACA91210.2816135179428659No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT88430.27303018738830864No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT75810.23406557170539047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG75350.23264530837621913No Hit
TTATTATTGTGTTCCAAACAGATTTTTGCAAGACTTCTGGGGCCCGGGAA74810.23097804272893102No Hit
GTCTCCGGGCACTGTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT73570.22714950679812132No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC71670.22128320174284838No Hit
GTGTATTACTGTCAGCAGTACGGTACGTCACGCATCACTTTCGGCGGAGG68840.21254549473946815No Hit
CATCTATGCTACCTCCAACAGGGCCACTGGCGTCCCAGACAGGTTCATGG68740.2122367418418222No Hit
CTCCGGGCACTGTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC66120.20414741592349844No Hit
ACCTCCAACAGGGCCACTGGCGTCCCAGACAGGTTCATGGGCAGTGTGTC64940.2005041317312763No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA62660.19346456566494877No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC62370.19256918226177555No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT60360.18636324901909204No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA60350.18633237372932746No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC60300.18617799728050446No Hit
CTACCTGGACTCCGTGAAGGGCCGGTTCACCATTTCCAGAGACAATTCCA60250.1860236208316815No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA60080.18549874090568338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA58630.1810218238898172No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC58460.1804969439638191No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT58260.17987943816852722No Hit
GTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGCGA57140.17642140571489262No Hit
GTACGGTACGTCACGCATCACTTTCGGCGGAGGGACCAGGGTGGAGAGCA56830.1754642717321902No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG56750.17521726941407345No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG54750.16904221146115456No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC53860.16629431067210565No Hit
CCCCCGGAGAGATTGTGTTGACGCAGTCTCCGGGCACTGTGTCTTTGTCT53350.16471967089411133No Hit
ACAATACACTGTATCTGGAGATGAACAGCCTGAGAGTCGAGGACACGGGC52330.1615703913381227No Hit
GTTCATGGGCAGTGTGTCTGGGACAGACTTCACTCTCACTATCAGCAGAC51100.1577727306970776No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC50870.15706259903249192No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATAGTGAGAGTGA50180.1549322040387349No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG49940.1541911970843846No Hit
GTATCTGGAGATGAACAGCCTGAGAGTCGAGGACACGGGCGTTTATTATT48990.15125804455674818No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT48510.14977603064804762No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG47150.1455769912400628No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT45210.13958718502573145No Hit
CAATAATAAACGCCCGTGTCCTCGACTCTCAGGCTGTTCATCTCCAGATA45210.13958718502573145No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45070.13915493096902715No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG44990.1389079286509104No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCACCCTGGTCC44920.13869180162255823No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA43700.1349250162712777No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT43520.134369261055515No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACC43410.1340296328681045No Hit
CACTATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGC43090.13304162359563745No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC43000.1327637459877561No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG42790.1321153649026996No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA42420.13097297918140963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC42420.13097297918140963No Hit
CCCCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTACCTCCAACAGGGCC41450.12797807607424397No Hit
GTCCCAGACAGGTTCATGGGCAGTGTGTCTGGGACAGACTTCACTCTCAC41400.12782369962542098No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT41270.12742232085848126No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCACCCTGGTCCCTCCGCCGAAAGTG41080.12683569035295397No Hit
TTCATGGGCAGTGTGTCTGGGACAGACTTCACTCTCACTATCAGCAGACT40870.12618730926789748No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG39790.12285277797332128No Hit
GTGTTGACGCAGTCTCCGGGCACTGTGTCTTTGTCTCCAGGGGAAAGAGC39170.12093851000791643No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTC39040.12053713124097669No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA38840.1199196254456848No Hit
ACTGTCAGCAGTACGGTACGTCACGCATCACTTTCGGCGGAGGGACCAGG38790.11976524899686185No Hit
GACTTCACTCTCACTATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA38570.11908599262204075No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTCCTATGTTTGCCATGAGTTGGG37840.11683209646922536No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCT37140.11467082618570376No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGAC36770.11352844046441375No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA36760.11349756517464918No Hit
TTCCTATGTTTGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGGCCG36600.11300356053841566No Hit
GGCACAAGCAGACCCCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTACC36170.1116759230785381No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGCGAGA36130.11155242191947971No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC35890.11081141496512945No Hit
ATGCTACCTCCAACAGGGCCACTGGCGTCCCAGACAGGTTCATGGGCAGT35820.1105952879367773No Hit
GTCCCAGACACACTGCCCATGAACCTGTCTGGGACGCCAGTGGCCCTGTT35310.10902064815878297No Hit
GTACCGTACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCT35300.10898977286901837No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC35220.10874277055090162No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA34700.10713725548314271No Hit
CTATTGGAGTCTGGTGGAGGCATGGTGCAGCCTGGGGGGTCCCTGAGACT34680.10707550490361352No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG34650.10698287903431974No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT34180.10553174041538381No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGA33800.1043584794043292No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG33790.10432760411456463No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT32820.10133270100739894No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT32750.10111657397904679No Hit
TTTAAAAGGTGTCCAGTGTGACGTGCAACTATTGGAGTCTGGTGGAGGCA32670.10086957166093004No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32660.10083869637116545No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG32480.10028294115540275No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTCCTATGTTTGCC32440.10015943999634436No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)