FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005113210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141566
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGA109990.3501120141992879No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT108590.34565563798436827No Hit
GTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACAATTGGATCCG107770.34304547477277253No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC98420.31328324790884543No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA90620.28845486614000787No Hit
GTATATTACTGTGCCAGAGGTGATACTAGTGGTTATTATCCCCTTTACTA77300.246055629580916No Hit
ATTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCC75520.24038966553623256No Hit
CAATAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGT62080.19760845387300474No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT58780.18710413850926574No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC56230.17898716754637656No Hit
GTATGGTAGTTCACCGTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA55100.17539023531576292No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT53890.17153865301572527No Hit
GAGTATTAGCAGCGGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG52540.1672414330941957No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50060.15934728094205247No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCT48270.15364948563869102No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA48210.15345849808662304No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47620.15158045382462124No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47270.15046635977089132No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT46830.14906578438905946No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA46570.14823817166343156No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC46540.14814267788739757No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGACATCCCAGACAGGTTCAGTG43690.13907076916416844No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43220.1375747000063026No Hit
CTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT42530.1353783431575208No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATT42390.13493270553602885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41340.13159042337483917No Hit
CACCTGCACTGTCTCAGGTGGCTCCATCAACAGTGGTGATTCCTACTACA41050.13066731687317726No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA40880.1301261854756513No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA39950.1271658784185976No Hit
ATTTTGTACAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGT38980.12407824632683191No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT38030.1210542767524222No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT37710.1200356764747263No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37150.11825312598875846No Hit
GTCCCAGGTGATGCTGCAGAGGTCGGGCCCAGGACTGGTGAAGCCTTCAC36770.11704353815899458No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT35200.11204603054654907No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA34970.11131391159695514No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC34470.10972234866305529No Hit
CTACCATACTGCTGACAATAATACACTGCAAAATCTGCAGGCTCCAGTCT34340.10930854230024134No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA34240.10899022971346138No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC33850.1077488106250195No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33300.10599809139772967No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC33200.10567977881094968No Hit
ATATACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACT32580.10370624077291389No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32140.10230566539108203No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)