FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005113242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3627175
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGGAGTTGAACAGCCTGATATCCGACGACACGGCCGTATATTA249460.687752865522066No Hit
GTTTTATACTCCTCCAACAATAAGAACTCCTTGGCTTGGTACCAGCAGAA149380.41183565722635385No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT143340.39518357950746796No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC119250.32876825628760675No Hit
CCATATATACCGTGCGTGTGGACATGTCCCTGGTCACGTTGAGTCTGTCG111850.30836670411546174No Hit
ATAATATACGGCCGTGTCGTCGGATATCAGGCTGTTCAACTCCATATATA73790.20343655875440253No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT71570.19731609310275905No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT71130.19610302783846933No Hit
ATATTATTGTGCGGCAGATTGGTTGATGCAGGGCACCTACTCTAACGTTA70460.19425586027693728No Hit
GTATATTATTGTGCGGCAGATTGGTTGATGCAGGGCACCTACTCTAACGT65690.18110513002543302No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG63380.1747365373879121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63060.1738543081047923No Hit
GTCATATTGGTGACAGAAGTAAACTGCAACATCTTCAGCCTGCAGGCTGC62560.17247582484991764No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTTCTGCA62040.171042202264848No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC59340.16359839268852483No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC58310.16075871718348303No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA57650.15893911928704846No Hit
CATATATACCGTGCGTGTGGACATGTCCCTGGTCACGTTGAGTCTGTCGT57530.15860828330587856No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA57260.1578639023482462No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT57090.15739521804158885No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55050.15177100636170024No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT52520.14479588109203445No Hit
CCTTTATTACGTCTGCTGTCCAGTGGGTGCGACAGGCTCGTGGACAACCC52260.1440790697994996No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG51610.14228704156816255No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA51380.1416529392709202No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA49370.13611143658632405No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG48940.13492594098713184No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT48410.13346474873696473No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48210.13291335543501487No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT47200.13012881926016803No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46810.1290536023213658No Hit
ATATGACAGTCCTCCTCGGACTTTTGGCCAGGGGACTAAGCTGGAGATCA46010.12684802911356635No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA45480.12538683686339921No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT44340.12224389504228497No Hit
CAATATGACAGTCCTCCTCGGACTTTTGGCCAGGGGACTAAGCTGGAGAT44070.12149951408465265No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC43920.12108596910819025No Hit
CTCCTAAACTCCTCATTTTCGGGGCATCTACCCGGGAATCCGGGGTCCCT42510.11719864632944371No Hit
ATATACGGCCGTGTCGTCGGATATCAGGCTGTTCAACTCCATATATACCG42070.115985581065154No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC40290.11107818067780022No Hit
ACTCTAACGTTATGGACGTCTGGGGTCAAGGGACCACGGTCATCGTCTCC39990.1102510907248754No Hit
GCCGTATATTATTGTGCGGCAGATTGGTTGATGCAGGGCACCTACTCTAA39880.109947824408803No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA39470.10881746813980576No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG38710.1067221735923963No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC38650.10655675560181131No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC37470.10330353512030713No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC37370.10302783846933221No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTAGTCC37360.1030002688042347No Hit
GTCCAGTGGGTGCGACAGGCTCGTGGACAACCCCTTGAATGGATGGGATG36570.10082226526153272No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTAGTCCCCTGGCCAAAAGTC36540.10073955626624025No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC36490.1006017079407528No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)