FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005113269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307986
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTACACACTCCTGCTGCTGACCTCCCCTTCCTGGGTCTTGTCCCAGG94610.2860048379890362No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC94310.2850979417687983No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC94230.28485610277673484No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT92380.2792635760852676No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA89350.2701039242608645No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC86050.2601280658382472No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA79940.2416576128194013No Hit
GACTTAGGACGGTGACCGTGGTCCCAGTTCCAAAGACCCCTGGGGTTCTT74780.22605899783130884No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT74620.22557531984718193No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA72600.21946888529757988No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT71670.21665750701484227No Hit
GCCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGAAGAACCC71560.216324978400755No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA71510.21617382903071536No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC71310.21556923155055674No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA70630.21351360011801743No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA69680.21064176208726396No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA69070.20879773977278016No Hit
ATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTACT68500.2070746369543281No Hit
GACCTGCACCGTCTCTGGGTTCTCACTCAGAAATCCTAGAATGGGTGTGA67970.20547245363190778No Hit
ACATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTA67400.20374935081345566No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT65900.19921486971226599No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA65740.19873119172813913No Hit
CCCCTGTGTTGGTCATCTATCAGGATAACAAGCGGCCCTCAGGGATCCCT65600.19830797349202808No Hit
TCCATAGGCTGGGTCCCGCTGATGGTCAGAGTGACTGTGTTCCCAGAGTT65040.19661510054758397No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC64650.1954361354612746No Hit
GTGTTGGTCATCTATCAGGATAACAAGCGGCCCTCAGGGATCCCTGAGCG64200.19407579113091772No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC62420.188694873557506No Hit
GACTATTACTGTCAGACGTGGGACAGAAGAACCCCAGGGGTCTTTGGAAC62180.18796935658131564No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG61860.18700200061306183No Hit
TCTTTGAAGAGCAGGCTCACCATCTTCAAGGACACCTCCAAAAGCCAGGT60820.18385809371623701No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC59700.18047234782734872No Hit
ATATAGTGGGAGCTCCTCTTTTGGCTACTGGGGCCAGGGAACCCTGGTCA59220.17902131387496803No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT56350.17034534003469182No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG56260.17007327116862042No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG55090.16653637590969247No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA53570.16194143506048697No Hit
GTCTCTGGGTTCTCACTCAGAAATCCTAGAATGGGTGTGAGCTGGATCCG51960.15707442534521004No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG51450.15553270177080555No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT49020.14818684238687832No Hit
CTCCTGCTGCTGACCTCCCCTTCCTGGGTCTTGTCCCAGGTCACCTTGCA48670.1471287967966007No Hit
CTATTACTGTCAGACGTGGGACAGAAGAACCCCAGGGGTCTTTGGAACTG48560.14679626818251348No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT46970.14198971821525241No Hit
CCCCAGGACAGACAGCCAGCATCTCCTGCTCTGGAGATGATTTGGGGGAT46860.14165718960116516No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT46610.14090144275096692No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC46430.14035730501882412No Hit
TAAATATGCTTCCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGTTGG45930.1388458113184276No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT45660.13802960472021344No Hit
CAGTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGAC44680.1350670770674362No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG44360.13409972109918242No Hit
GGATAACAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACT44330.13400903147715862No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG43620.1318627104225955No Hit
GTAATAGTCAGCCTCATCCATAGGCTGGGTCCCGCTGATGGTCAGAGTGA43300.13089535445434172No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG43130.13038144659620687No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC42520.1285374242817231No Hit
GCCCTGGAGTGGCTTGCACACATTTTTTCGAATGACGAAAAATCCTACAG42020.12702593058132652No Hit
CATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTAC42000.12696547083331067No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC40610.12276351834620824No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG40450.12227984036208134No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39900.12061719729164512No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC39820.1203753582995817No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT39500.11940800233132788No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG39430.11919639321327237No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCTCCTGCTCTGG39410.11913593346525651No Hit
ACATGGACCCTGTGGACACAGCCACATATTACTGTGCACGGGAGCGATAT38200.11547811871029684No Hit
GTATCAACGCAGAGTACGGGCTGGGCCTCAGGAAGCAGCATCGGAGGTGC38180.11541765896228098No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37910.11460145236406685No Hit
GTCTGGTCCTGTGCTGATGAAACCCACAGAGACCCTCACTCTGACCTGCA37410.11308995866367029No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA37310.11278765992359097No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA36860.11142731559323407No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTC36570.11055064924700407No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG36010.10885777630255991No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT35590.10758812159422683No Hit
CAGTCACTCTGACCATCAGCGGGACCCAGCCTATGGATGAGGCTGACTAT35520.1073765124761713No Hit
GAAGAACCCCAGGGGTCTTTGGAACTGGGACCACGGTCACCGTCCTAAGT34900.10550226028767956No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG34880.1054418005396637No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC34870.10541157066565578No Hit
GGATGAGGCTGACTATTACTGTCAGACGTGGGACAGAAGAACCCCAGGGG34740.10501858230355268No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG34120.10314433011506094No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG33730.10196536502875163No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC33660.10175375591069613No Hit
CTTTGGAACTGGGACCACGGTCACCGTCCTAAGTCAGCCCAAGGCCAACC33400.1009677791864899No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)