FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005113270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307986
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTACACACTCCTGCTGCTGACCTCCCCTTCCTGGGTCTTGTCCCAGG103850.3139372415723646No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT96810.29265541027078107No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA90700.27418495725193515No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT87100.26330220260908No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC86950.262848754498961No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT85030.25704461868943823No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC80120.24220175055154405No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA78780.23815094743448129No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC77000.23277002986106954No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA74940.22654267581543575No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC72080.21789693184916742No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA71530.21623428877873122No Hit
GACTTAGGACGGTGACCGTGGTCCCAGTTCCAAAGACCCCTGGGGTTCTT69890.21127658944143052No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA68580.20731647594639155No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA68000.20556314325393155No Hit
TCCATAGGCTGGGTCCCGCTGATGGTCAGAGTGACTGTGTTCCCAGAGTT67460.20393073005750326No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC66800.2019355583729798No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT65320.197461537019806No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT64920.19625234205948877No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA64270.19428740024897323No Hit
GCCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGAAGAACCC63480.1918992402023467No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG62900.1901459075098867No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA62500.18893671254956945No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA60350.18243728963786424No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG59950.181228094677547No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC58810.17778188904064285No Hit
ATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTACT58680.17738890067853977No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT58660.1773284409305239No Hit
ACATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTA57910.17506120037992906No Hit
CCCCTGTGTTGGTCATCTATCAGGATAACAAGCGGCCCTCAGGGATCCCT57590.17409384441167525No Hit
GACCTGCACCGTCTCTGGGTTCTCACTCAGAAATCCTAGAATGGGTGTGA56900.17200798310512802No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG56750.17155453499500906No Hit
GACTATTACTGTCAGACGTGGGACAGAAGAACCCCAGGGGTCTTTGGAAC55190.1668386746497718No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT54800.1656597095634625No Hit
TCTTTGAAGAGCAGGCTCACCATCTTCAAGGACACCTCCAAAAGCCAGGT54370.16435982498112145No Hit
GTGTTGGTCATCTATCAGGATAACAAGCGGCCCTCAGGGATCCCTGAGCG53380.16136706745433627No Hit
CTCCTGCTGCTGACCTCCCCTTCCTGGGTCTTGTCCCAGGTCACCTTGCA53170.1607322401001697No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC51520.15574431088886107No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT51230.15486764454263108No Hit
ATATAGTGGGAGCTCCTCTTTTGGCTACTGGGGCCAGGGAACCCTGGTCA50680.15320500147219485No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA47470.143501211915649No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC45370.13715293837398343No Hit
CTATTACTGTCAGACGTGGGACAGAAGAACCCCAGGGGTCTTTGGAACTG44910.1357623641696186No Hit
GTCTCTGGGTTCTCACTCAGAAATCCTAGAATGGGTGTGAGCTGGATCCG44800.13542983555553137No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG43900.13270914689481758No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT42910.1297163893680324No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC42480.12841650478569136No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC42380.12811420604561205No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT42240.127690987809501No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG41840.12648179284918376No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT41670.12596788499104894No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC41360.12503075889680307No Hit
GTAATAGTCAGCCTCATCCATAGGCTGGGTCCCGCTGATGGTCAGAGTGA41060.12412386267656512No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG40960.12382156393648583No Hit
TAAATATGCTTCCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGTTGG40790.123307656078351No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG40510.12246121960612893No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40370.12203800137001788No Hit
GTCTGGTCCTGTGCTGATGAAACCCACAGAGACCCTCACTCTGACCTGCA40320.12188685199997823No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG38860.1174732903948203No Hit
CCCCAGGACAGACAGCCAGCATCTCCTGCTCTGGAGATGATTTGGGGGAT38470.11629432530851097No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT38350.1159315668204158No Hit
CAGTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGAC38170.11538742908827306No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC37680.11390616526188442No Hit
GGATAACAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACT37050.11200168319938476No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC36780.11118547660117062No Hit
CATATTACTGTGCACGGGAGCGATATAGTGGGAGCTCCTCTTTTGGCTAC36740.1110645571051389No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG36640.1107622583650596No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCTCCTGCTCTGG35890.10849501781446476No Hit
GTATCAACGCAGAGTACGGGCTGGGCCTCAGGAAGCAGCATCGGAGGTGC35830.10831363857041716No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG35510.10734628260216338No Hit
GCCCTGGAGTGGCTTGCACACATTTTTTCGAATGACGAAAAATCCTACAG35490.1072858228541475No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG35330.1068021448700206No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTC35210.10643938638192545No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA35070.10601616814581441No Hit
ACATGGACCCTGTGGACACAGCCACATATTACTGTGCACGGGAGCGATAT35060.10598593827180647No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG34870.10541157066565578No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG34600.10459536406744163No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT34160.10326524961109267No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT33360.10084685969045817No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)