FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005113303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207159
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC187060.5832576432911496No Hit
GTAATACACAGCCGCGTCCGCGGCGGTCACAGAGTATAACATCAGGGAGA166960.5205853529556844No Hit
GTGTATTACTGTGCGAGAGGGTGGACGATTTTTGGAGCGGTAAGATGCTT150270.4685455258064848No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT144040.44912023382688543No Hit
ATGTTATACTCTGTGACCGCCGCGGACGCGGCTGTGTATTACTGTGCGAG125970.39277753301286283No Hit
GTATTACTGTCAGCAGTATGGTAGCTCAAGGATGTACACTTTTGGCCAGG119480.3725415546906156No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC94370.29424796213720616No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT86540.26983383112592796No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT86320.2691478657590721No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC85700.2672146906342966No Hit
CTCTATTGCAGGGCCAGTCAGAGTGTCAGCAGCGCCTACTTAGCCTGGTA85080.26528151550952106No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC82000.2556780003735393No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTATT81990.25564682012959133No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT81510.2541501684200877No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA80350.25053326012212057No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC74180.23129504960620909No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG73980.23067144472724926No Hit
TCACTAGACACGTCCAAGAACCAGTTCTCCCTGATGTTATACTCTGTGAC72700.2266803735019062No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA72690.22664919325795818No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT65890.20544662737332323No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC65230.20338873127275572No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC64820.20211034127088803No Hit
GTTATACTCTGTGACCGCCGCGGACGCGGCTGTGTATTACTGTGCGAGAG62900.19612373443287345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT62620.19525068760232966No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA61950.19316161125781417No Hit
CTATTGCAGGGCCAGTCAGAGTGTCAGCAGCGCCTACTTAGCCTGGTACC61710.19241328540306235No Hit
GTATAACATCAGGGAGAACTGGTTCTTGGACGTGTCTAGTGATATGGTGA61160.19069837198592274No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC59970.18698792295611164No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG56240.1753576919635104No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT56200.17523297098771842No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA56030.17470290684060255No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT54290.16927754439365184No Hit
GTATTACTGTGCGAGAGGGTGGACGATTTTTGGAGCGGTAAGATGCTTCC52720.164382246093817No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC50570.15767849364499859No Hit
CCATATCACTAGACACGTCCAAGAACCAGTTCTCCCTGATGTTATACTCT50120.1562753826673389No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG49780.15521525437310718No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT49520.15440456803045938No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA49490.15431102729861537No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT49450.1541863063228234No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG48590.15150480534329605No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA47730.14882330436376867No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTATTGC47090.14682776875109715No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT46740.14573646021291742No Hit
GTCCTGGCCCAGATGCAGCTACACCAGTGGGGCGCAGGACTGTTGAAGCC46260.14423980850341375No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCAAGGATGTACACTTTTGGCCA46050.14358502338050594No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG45720.14255607533022216No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC45030.14040463849781068No Hit
GTCCATCAGTGCTTACTACTGGAGCTGGATCCGCCAGTCCCCACAGAAGG44260.13800375971381526No Hit
TCTACGATGCATCCAGTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC44160.13769195727433534No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG44150.13766077703038734No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT43990.13716189312721946No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG43940.13700599190747947No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG42660.13301492068213644No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTATTGCAGGGCCAGTCAGA42590.1327966589745005No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA41090.1281196223823016No Hit
GAGTATTATCAGCTACTTTGCCTGGTACCCACAGAAACCTGGCCAGGCTC40850.12737129652754978No Hit
CAGTAATACACAGCCGCGTCCGCGGCGGTCACAGAGTATAACATCAGGGA40260.1255316621346182No Hit
GTGCTTACTACTGGAGCTGGATCCGCCAGTCCCCACAGAAGGGGCTGGAG40020.12478333627986637No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC39780.12403501042511456No Hit
CTACTGGAGCTGGATCCGCCAGTCCCCACAGAAGGGGCTGGAGTGGATTG39550.12331786481431073No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG39370.12275662042324686No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA39350.12269425993535088No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39280.12247599822771493No Hit
TCTCTGATGCATCCAACAGGGCCACTGGCATCACAGCCAGGTTCAGTGGC39260.12241363773981895No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38670.12057400334688738No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC38260.11929561334501969No Hit
TCCTTGAGCTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGC38170.11901499114948776No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG38160.11898381090553976No Hit
GTTTACACCTGTCAGCAGTGTAGCAACTGGGGCACCTTCGGCCAAGGGAC37340.11642703090180435No Hit
GTATGGTAGCTCAAGGATGTACACTTTTGGCCAGGGGACCAAGCTGGAGA36970.11527336187572865No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT36960.11524218163178065No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA36200.11287248309173321No Hit
GTGCTGGAAGCATCTTACCGCTCCAAAAATCGTCCACCCTCTCGCACAGT36090.1125295004083053No Hit
GTCCCTCACCTGCGGTGTCGATGGTGGGTCCATCAGTGCTTACTACTGGA35870.11184353504144946No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA35720.11137583138222956No Hit
GTTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGGTGTCGATGGTGGGT35690.1112822906503856No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC35680.11125111040643759No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA35650.11115756967459361No Hit
CCTCATCTACGATGCATCCAGTAGGGCCACTGGCATCCCAGACAGGTTCA35430.11047160430773778No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC35380.11031570308799783No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG35330.11015980186825786No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT34940.10894377235428615No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG34310.10697941698556261No Hit
CAGCTACACCAGTGGGGCGCAGGACTGTTGAAGCCTTCGGAGACCCTGTC34200.1066364343021347No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC33760.10526450356842301No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA33620.10482798015315112No Hit
GAGATATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGG33100.10320660746785551No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG33080.10314424697995953No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC32590.10161641502650788No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT32480.10127343234307996No Hit
CAGTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGA32470.10124225209913197No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG32370.10093044965965206No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC32270.10061864722017211No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)