FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005113381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527773
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT44630.17655857547335144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41660.16480910271610624No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT32940.13031233421671962Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT26660.10546833121486779No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC26510.10487492349985542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)