FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005113454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264895
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC60250.2660167469132123No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA56600.2499012095483455No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51870.2290172392097647No Hit
GGGTTAACAGTGATGGCAGCCACAACAGGGGGGACGAGATCCCTGATCGC51680.22817834822364833No Hit
CCACTACACAGGGTCTCTCTCCCAACTTGTGTTGACTCAATCGCCCTCTG50650.22363067603575443No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47990.21188620223012547No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC46290.2043803355122423No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT46100.20354144452612594No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA45690.2017312060824012No Hit
ACACAGGGTCTCTCTCCCAACTTGTGTTGACTCAATCGCCCTCTGCCTCT43620.1925917095494493No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG41990.1853949079317143No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC40780.18005249691486802No Hit
CTATTATTGTCAGACCTGGGACACTGGGCTTCGAGTCTTCGGCGGAGGGA40580.17916945377158766No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT39250.17329721686877317No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37860.16716006702297456No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA37120.16389280739283718No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37070.16367204660701712No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG37050.16358374229268904No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA36200.1598308089337475No Hit
GTCTACATGGAGCTGAGGCGCCTGAGAACTGACGACACGGCCCTGTATTT35690.15757904891838254No Hit
GTCTGGACCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA35470.15660770146077413No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35210.15545974537450963No Hit
CCATTAAGCAGTTCGTACGACTCATCTCTCGAACAGAAATACAGGGCCGT34350.15166265985840402No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC33880.14958750847169516No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACTCGAAGCCCAGTG33190.14654100962737787No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACTCG30300.13378103620697648No Hit
GTTCTGGGGCTGAGCGCTACCTCATGATCTCCAGCCTCCAGTCTGAGGAT30130.13303044953518817No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT29930.1321474063919078No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT29820.1316617326631036No Hit
CTCCCAACTTGTGTTGACTCAATCGCCCTCTGCCTCTGCCTCTTTGGGAG29660.13095529814847928No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA29450.1300281028480349No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29330.12949827696206667No Hit
ATTATTGTCAGACCTGGGACACTGGGCTTCGAGTCTTCGGCGGAGGGACC29310.12940997264773865No Hit
ATACAGGGCCGTGTCGTCAGTTCTCAGGCGCCTCAGCTCCATGTAGACTG28870.1274672777325218No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG28640.12645177811774938No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC28510.12587780007461716No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA27720.12238977965865966No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG27600.12185995377269146No Hit
TCCTCACCCTCCTCCTCCACTACACAGGGTCTCTCTCCCAACTTGTGTTG27340.12071199768642696No Hit
GCTTAATGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT27340.12071199768642696No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTTTCGATGGT27040.1193874329715064No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26690.11784210747076576No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA26600.11744473805628958No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT26580.11735643374196154No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC26090.11519297804092464No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG25880.11426578274048024No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25720.11355934822585595No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG25510.11263215292541155No Hit
CTATTTGATGAGGGTTAACAGTGATGGCAGCCACAACAGGGGGGACGAGA24820.10958565408109427No Hit
CTGTTAACCCTCATCAAATAGCGAGGGCCCTTCTCTGGCTGCTGCTGAAG24800.10949734976676623No Hit
CTCCCAGGTTCAGCTGGTGCAGTCTGGACCTGAGGTGAAGAAGCCTGGGG24690.10901167603796202No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24590.10857015446632183No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24300.1072897419085653No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTG24150.10662745955110503No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCC24150.10662745955110503No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23730.10477306895021624No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA23620.10428739522141202No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG23500.1037575693354438No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT23400.10331604776380363No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG23250.10265376540634333No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA23190.10238885246335924No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA22860.1009318312769466No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)