FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005113525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363484
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189571.3903353468027493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91720.6726884950611814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89030.6529596240219907No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCG69330.5084768138093296TruSeq Adapter, Index 6 (96% over 33bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG69010.5061298849124742No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA67060.4918282869472616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG60940.44694327179490184No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA55730.40873233569297474No Hit
TATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA49500.363040563732321TruSeq Adapter, Index 16 (97% over 34bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT38980.2858852762481994No Hit
GTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCACC37140.27239043509128086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG35490.2602890829668702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG35330.25911561851844245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG34660.25420173614065145No Hit
GTATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCC31610.23183257009249833TruSeq Adapter, Index 1 (96% over 32bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA28480.20887667182013137No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT27520.20183588512956516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA27340.20051573762508396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27160.19919559012060278No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG26860.19699534427980087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT26420.19376831704662467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT26170.1919347788459564No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA25160.18452728451525652No Hit
GTATCAACGCAGAGTACGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA24320.17836659616101105TruSeq Adapter, Index 16 (97% over 37bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA23990.17594632573612892No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23130.16963895432583No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGT22620.1658985363964667No Hit
GGTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCC22590.16567851181238652TruSeq Adapter, Index 6 (96% over 32bp)
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA22250.16318489985947762No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA21720.15929779887406087No Hit
TATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCCG21120.15489730719245698TruSeq Adapter, Index 1 (96% over 33bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG20950.15365050121600254No Hit
GAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCA20830.15277040287968174Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT20720.1519636460713877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG19780.14506954243687495No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA19710.14455615174068784No Hit
TATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGG18790.13780873116222853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT18690.1370753158819612No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGTCTT18690.1370753158819612No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT17800.1305479198875821Illumina PCR Primer Index 7 (95% over 22bp)
TATCAACGCAGAGTACGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGAT16860.12365381625306934TruSeq Adapter, Index 16 (100% over 36bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA16320.11969337373962585No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAGCAGAAGACGGCATA14840.10883882759166957No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA14790.10847211995153591No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT14250.10451167743809242No Hit
CTGGAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAG14150.1037782621578251Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA13890.10187138242913009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)