FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005113565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60561855
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA39450.030.91128732
TCGTATG49050.024.8157544
CGTATGC58800.020.81946645
TCACGCG63000.019.57063129
ACGCGCA66100.018.51664231
CGCAAGC67250.018.09963434
CTCGTAT71150.017.29741543
ATCGGAA83000.016.1393741
CGTATCG43900.015.3812723
GTATCGA46850.014.5511684
CCCGTAT60400.014.4624751
GCGCAAG85050.014.36449433
ACACGTC90600.014.03117612
AGTCACG91100.013.83039127
CACGCGC96350.013.05343830
CCGTATC58200.012.9555942
ACGTCTG98400.012.91894814
TCGGAAG123550.012.9163272
GTCACGC100400.012.57169828
TATCGAA57200.012.2328935
Produced by FastQC (version 0.11.5)