FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005113571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005113571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62784891
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA74650.018.3717484
CCCGTAT93150.017.785381
TATCGAA80700.017.4123335
CGTATCG93450.015.9028913
CCGTATC112300.013.8835132
TCGTATG39050.013.49576444
ATCGAAG134150.012.8544996
CTCCGAT91800.012.7361151
CGCGTAT6750.012.0378411
ACCGAAT53250.011.703242
CGCGTTA40950.011.47950334
CGGAGCG50250.010.96518732
CGTATGC48950.010.85909145
CGCTAGG20700.010.6857651
GTTACGG46250.010.597577
CACCGAA68350.010.5342121
GTCGCGT45800.010.06640632
CCGGTAA49700.09.9457521
CTCCGTT79550.09.9306521
TCCGATT91350.09.8513872
Produced by FastQC (version 0.11.5)